Human ABCG2 (ATP Binding Cassette Transporter G2) CLIA Kit | ABCG2 clia kit

Human ABCG2 (ATP Binding Cassette Transporter G2) CLIA Kit

Cat. Num. AAA2532868

• Gene Names: ABCG2; MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
• Reactivity: Human
• Synonyms: ABCG2 (ATP Binding Cassette Transporter G2); Human ABCG2 (ATP Binding Cassette Transporter G2) CLIA Kit; ABCG2 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant ABCG2. No significant cross-reactivity or interference between ABCG2 and analogues was observed.
• Sequence Length: 655

• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCG2 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of ABCG2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to ABCG2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ABCG2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ABCG2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of ABCG2. You can calculate the concentration of ABCG2 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62526033
• NCBI GeneID: 9429
• NCBI Accession #: NP_004818.2
• NCBI GenBank Nucleotide #: NM_004827.2
• Molecular Weight: 67,453 Da
• NCBI Official Full Name: ATP-binding cassette sub-family G member 2 isoform 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group)
• NCBI Official Symbol: ABCG2
• NCBI Official Synonym Symbols: MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
• NCBI Protein Information: ATP-binding cassette sub-family G member 2
• UniProt Protein Name: ATP-binding cassette sub-family G member 2
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG2
• UniProt Synonym Gene Names: ABCP; BCRP; BCRP1; MXR
• UniProt Entry Name: ABCG2_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

ABCG2: Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123. Monomer or homodimer; disulfide-linked. Up-regulated in brain tumors. Highly expressed in placenta. Low expression in small intestine, liver and colon. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, iron; Transporter, ABC family

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: nucleoplasm; mitochondrial membrane; integral to membrane; plasma membrane; nucleus

Molecular Function: protein binding; protein homodimerization activity; transporter activity; ATPase activity, coupled to transmembrane movement of substances; heme transporter activity; ATP binding; xenobiotic-transporting ATPase activity

Biological Process: response to drug; heme transport; urate metabolic process; cellular iron ion homeostasis; transport; multidrug transport; xenobiotic transport; transmembrane transport

Disease: Blood Group, Junior System; Uric Acid Concentration, Serum, Quantitative Trait Locus 1