Rabbit anti-Human PHPT1 Polyclonal Antibody | anti-PHPT1 antibody

Anti-PHPT1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106466

• Gene Names: PHPT1; PHP14; CGI-202; HSPC141; HEL-S-132P
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PHPT1; Polyclonal Antibody; Anti-PHPT1 Antibody; Rabbit Polyclonal; PHPT1 Antibody; Rabbit PAb; Antigen Affinity Purified; phosphohistidine phosphatase 1; Anti-CGI-202 Antibody; Anti-HEL-S-132P Antibody; Anti-HSPC141 Antibody; Anti-PHP14 Antibody; anti-PHPT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PHPT1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PHPT1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human PHPT1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human PHPT1 (rh PHPT1). PHPT1 specific IgG was purified by Human PHPT1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human PHPT1 in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(PHPT1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg THP-1 Whole Cell LysateLane D:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PHPT1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PHPT1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size: 14 kDa)

Western Blot (WB)

(Anti-PHPT1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)

Related Product Information for anti-PHPT1 antibody

PHPT1, also known as 14 kDa phosphohistidine phosphatase, phosphohistidine phosphatase 1, protein janus-A homolog, PHP14, is a cytoplasm protein which belongs to the janus family. PHPT1/PHP14 is expressed abundantly in heart and skeletal muscle. Phosphatases are a diverse group of enzymes that regulate numerous cellular processes. Much of what is known relates to the tyrosine, threonine, and serine phosphatases, whereas the histidine phosphatases have not been studied as much. Protein histidine phosphorylation exists widely in vertebrates, and it plays important roles in signal transduction and other cellular functions. Protein histidine phosphorylation accounts for about 6% of the total protein phosphorylation in eukaryotic cells. The knowledge about eukaryotic PHPT (protein histidine phosphatase) is still very limited. To date, only one vertebrate PHPT has been discovered, and two crystal structures of human PHPT1 have been solved. PHPT1/PHP14 can dephosphorylate a variety of proteins (e.g. ATP-citrate lyase and the beta-subunit of G proteins). A putative active site has been identified by its electrostatic character, ion binding, and conserved protein residues.

References

Busam, R.D. et al., 2006, J Biol Chem. 281 (45):33830-4. Zhang, X.Q. et al., 2009, Ups J Med Sci.114 (2):65-72. Gong, W. et al., 2009, Biochem J. 418 (2):337-44. Chapin, L.J. et al., 2009, J Exp Bot. 60 (7):2179-90.

NCBI and Uniprot Product Information

• NCBI GI #: 25008934
• NCBI GeneID: 29085
• UniProt Accession #: Q9NRX4; Q9H0Y3; B1AMX0; B1AMX1
• Molecular Weight: 125
• NCBI Official Full Name: 14 kDa phosphohistidine phosphatase
• NCBI Official Synonym Full Names: phosphohistidine phosphatase 1
• NCBI Official Symbol: PHPT1
• NCBI Official Synonym Symbols: PHP14; CGI-202; HSPC141; HEL-S-132P
• NCBI Protein Information: 14 kDa phosphohistidine phosphatase; protein janus-A homolog; sex-regulated protein janus-a; epididymis secretory sperm binding protein Li 132P
• UniProt Protein Name: 14 kDa phosphohistidine phosphatase
• Protein Family: 14 kDa phosphohistidine phosphatase
• UniProt Gene Name: PHPT1
• UniProt Synonym Gene Names: PHP14
• UniProt Entry Name: PHP14_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.

Protein type: Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.-; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol

Molecular Function: phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation