PHPT1 / PHP14 recombinant protein

Recombinant Human PHPT1 / PHP14 Protein (His tag)

Cat. Num. AAA2545502

• Gene Names: PHPT1; PHP; PHP14; CGI-202; HSPC141; HEL-S-132P
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: PHPT1 / PHP14; Recombinant Human PHPT1 / PHP14 Protein (His tag); PHPT1; PHP14; PHPT1 / PHP14 recombinant protein

• Host: E Coli
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 124

• Application Notes: The recombinant human PHPT1 consisting of 135 amino acids and has a calculated molecular mass of 15.2 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PHPT1 / PHP14 recombinant protein

Background: PHPT1, also known as 14 kDa phosphohistidine phosphatase, phosphohistidine phosphatase 1, protein janus-A homolog, PHP14, is a cytoplasm protein which belongs to the janus family. PHPT1 / PHP14 is expressed abundantly in heart and skeletal muscle. Phosphatases are a diverse group of enzymes that regulate numerous cellular processes. Much of what is known relates to the tyrosine, threonine, and serine phosphatases, whereas the histidine phosphatases have not been studied as much. Protein histidine phosphorylation exists widely in vertebrates, and it plays important roles in signal transduction and other cellular functions. Protein histidine phosphorylation accounts for about 6% of the total protein phosphorylation in eukaryotic cells. The knowledge about eukaryotic PHPT (protein histidine phosphatase) is still very limited. To date, only one vertebrate PHPT has been discovered, and two crystal structures of human PHPT1 have been solved. PHPT1 / PHP14 can dephosphorylate a variety of proteins (e.g. ATP-citrate lyase and the beta-subunit of G proteins). A putative active site has been identified by its electrostatic character, ion binding, and conserved protein residues.

Description: A DNA sequence encoding the human PHPT1 (Q9NRX4-1) (Ala 2-Tyr 125) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 209529723
• NCBI GeneID: 29085
• NCBI Accession #: NP_001129333.1
• NCBI GenBank Nucleotide #: NM_001135861.2
• UniProt Accession #: Q9NRX4; Q9H0Y3; B1AMX0; B1AMX1
• Molecular Weight: 13,672 Da
• NCBI Official Full Name: 14 kDa phosphohistidine phosphatase isoform 2
• NCBI Official Synonym Full Names: phosphohistidine phosphatase 1
• NCBI Official Symbol: PHPT1
• NCBI Official Synonym Symbols: PHP; PHP14; CGI-202; HSPC141; HEL-S-132P
• NCBI Protein Information: 14 kDa phosphohistidine phosphatase
• UniProt Protein Name: 14 kDa phosphohistidine phosphatase
• UniProt Gene Name: PHPT1
• UniProt Synonym Gene Names: PHP14; PHP

NCBI Description

This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.

Protein type: Carbohydrate Metabolism - fructose and mannose; Cofactor and Vitamin Metabolism - riboflavin; Cofactor and Vitamin Metabolism - thiamine; EC 3.1.3.-; Motility/polarity/chemotaxis; Phosphatase

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol

Molecular Function: calcium channel inhibitor activity; phosphohistidine phosphatase activity

Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation