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Western Blot (WB) (Western Blot analysis of PHPT1 in 231 cell lysate using PHPT1 Rabbit pAb at dilution 1/800)

Rabbit anti-Human, Mouse PHPT1 Polyclonal Antibody | anti-PHPT1 antibody

PHPT1 Rabbit pAb

Gene Names
PHPT1; PHP14; CGI-202; HSPC141; HEL-S-132P
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
PHPT1; Polyclonal Antibody; PHPT1 Rabbit pAb; phosphohistidine phosphatase 1; PHP; PHP14; CGI-202; HSPC141; HEL-S-132P; anti-PHPT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
1.5mg/ml (varies by lot)
Applicable Applications for anti-PHPT1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:1000-1:5000
IHC-P: 1:200-1:300
Immunogen
Fusion protein of human PHPT1
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of PHPT1 in 231 cell lysate using PHPT1 Rabbit pAb at dilution 1/800)

Western Blot (WB) (Western Blot analysis of PHPT1 in 231 cell lysate using PHPT1 Rabbit pAb at dilution 1/800)

Immunohistochemistry (IHC)

(Immunohistochemistry of PHPT1 in paraffin-embedded Human colorectal cancer tissue using PHPT1 Rabbit pAb at dilution 1/170. Predicted cell location: Cytoplasm)

Immunohistochemistry (IHC) (Immunohistochemistry of PHPT1 in paraffin-embedded Human colorectal cancer tissue using PHPT1 Rabbit pAb at dilution 1/170. Predicted cell location: Cytoplasm)
Related Product Information for anti-PHPT1 antibody
This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
125
NCBI Official Full Name
14 kDa phosphohistidine phosphatase
NCBI Official Synonym Full Names
phosphohistidine phosphatase 1
NCBI Official Symbol
PHPT1
NCBI Official Synonym Symbols
PHP14; CGI-202; HSPC141; HEL-S-132P
NCBI Protein Information
14 kDa phosphohistidine phosphatase; protein janus-A homolog; sex-regulated protein janus-a; epididymis secretory sperm binding protein Li 132P
UniProt Protein Name
14 kDa phosphohistidine phosphatase
UniProt Gene Name
PHPT1
UniProt Synonym Gene Names
PHP14
UniProt Entry Name
PHP14_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

PHPT1: a phosphohistidine phosphatase. Present preferentially in heart and skeletal muscle. Insensitive to inhibition by okadaic acid and EDTA.

Protein type: Carbohydrate Metabolism - fructose and mannose; Phosphatase; Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.-; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - thiamine

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytosol

Molecular Function: phosphohistidine phosphatase activity; calcium channel inhibitor activity; phosphoprotein phosphatase activity

Biological Process: negative regulation of lyase activity; negative regulation of T cell receptor signaling pathway; protein amino acid dephosphorylation

Research Articles on PHPT1

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Product Notes

The PHPT1 phpt1 (Catalog #AAA4760460) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHPT1 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PHPT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:1000-1:5000 IHC-P: 1:200-1:300. Researchers should empirically determine the suitability of the PHPT1 phpt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHPT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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