Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SIGIRR expression on SW480 cells. Cells were stained with purified anti-Human SIGIRR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Human SIGIRR Monoclonal Antibody | anti-SIGIRR antibody
Recombinant Anti-SIGIRR Antibody, Rabbit Monoclonal
Gene Names
SIGIRR; TIR8
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
SIGIRR; Monoclonal Antibody; Recombinant Anti-SIGIRR Antibody; Rabbit Monoclonal; SIGIRR/TIR8 Antibody; Rabbit MAb; single immunoglobulin and toll-interleukin 1 receptor (TIR) domain; Anti-TIR8 Antibody; anti-SIGIRR antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
010
Specificity
Human SIGIRR/TIR8
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SIGIRR antibody
Flow Cytometry (FC/FACS/FCM)
Application Notes
FC/FACS/FCM: 1:25-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human SIGIRR/TIR8 Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SIGIRR/TIR8 (rh SIGIRR/TIR8).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SIGIRR expression on SW480 cells. Cells were stained with purified anti-Human SIGIRR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SIGIRR expression on SW480 cells. Cells were stained with purified anti-Human SIGIRR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-SIGIRR antibody
Single Ig IL-1-related receptor (SIGIRR) or TIR8 is a member of Toll-like receptor-interleukin 1 receptor signaling (TLR-IL-1R) receptor superfamily. Although SIGIRR/TIR8 shows the typical conserved motifs that characterize the IL-1R and Toll superfamily, it is structurally and functionally distinct from both. SIGIRR/TIR8 has only one Ig domain in its extracellular portion whereas the IL-1R family contains three Ig folds. An unusually long cytoplasmic domain is reminiscent of the structure of drosophila Toll, yet the SIGIRR peptide sequence is more closely related to IL-1RI. SIGIRR/TIR8 was mainly expressed in mouse and human epithelial tissues such as kidney, lung and gut. Resting and activated T and B lymphocytes and monocytes-macrophages expressed little or no SIGIRR/TIR8, with the exception of the mouse GG2EE macrophage line. Inflammation is enhanced in SIGIRR-deficient mice. SIGIRR negatively modulates immune responses. Inflammation is enhanced in SIGIRR-deficient mice, as shown by their enhanced chemokine induction after IL-1 injection and reduced threshold for lethal endotoxin challenge.
References
Wald D, et al. (2003) SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat Immunol. 4(9): 920-7.Polentarutti N, et al. (2003) Unique pattern of expression and inhibition of IL-1 signaling by the IL-1 receptor family member TIR8/SIGIRR. Eur Cytokine Netw. 14(4): 211-8.Wald D, et al. (2003) SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat Immunol. 4(9): 920-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted MW: 45 kDa
Observed MW: 45 kDa
Observed MW: 45 kDa
NCBI Official Full Name
SIGIRR
NCBI Official Synonym Full Names
single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
NCBI Official Symbol
SIGIRR
NCBI Official Synonym Symbols
TIR8
NCBI Protein Information
single Ig IL-1-related receptor; toll/interleukin-1 receptor 8; single Ig IL-1R-related molecule; single immunoglobulin domain IL1R1 related; single immunoglobulin domain-containing IL1R-related protein
UniProt Protein Name
Single Ig IL-1-related receptor
Protein Family
UniProt Gene Name
SIGIRR
UniProt Synonym Gene Names
TIR8
UniProt Entry Name
SIGIR_HUMAN
Uniprot Description
SIGIRR: Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP. Belongs to the interleukin-1 receptor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: membrane; integral to membrane
Molecular Function: protein binding
Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of transcription factor activity; acute-phase response; negative regulation of cytokine and chemokine mediated signaling pathway; signal transduction; negative regulation of chemokine biosynthetic process
Research Articles on SIGIRR
Similar Products
Product Notes
The SIGIRR sigirr (Catalog #AAA8106233) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-SIGIRR Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIGIRR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the SIGIRR sigirr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGIRR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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