Western Blot (WB)
(Western blot analysis of SIGIRR expression in (A) A549 and (B) MCF7 cell lysate with SIGIRR antibody at 1 μg/ml.)
Rabbit anti-Human, Mouse SIGIRR Polyclonal Antibody | anti-SIGIRR antibody
SIGIRR Antibody
Gene Names
SIGIRR; TIR8
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
SIGIRR Antibody is affinity chromatography purified via peptide column.
Synonyms
SIGIRR; Polyclonal Antibody; SIGIRR Antibody; SIGIRR Antibody: TIR8; Single Ig IL-1-related receptor; Single Ig IL-1R-related molecule; TIR8; anti-SIGIRR antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SIGIRR Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; SIGIRR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
410
Applicable Applications for anti-SIGIRR antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
SIGIRR antibody can be used for the detection of SIGIRR by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.
Optimal dilutions for each application to be determined by the researcher.
Optimal dilutions for each application to be determined by the researcher.
Immunogen
SIGIRR antibody was raised against a synthetic peptide corresponding to 15 amino acids at the C-terminus of human SIGIRR. The immunogen is located within the last 50 amino acids of SIGIRR.
Homology
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Conjugate
Unconjugated
Positive Control
MBS151733 - MCF Cell Lysate
MBS154287 - A549 Cell Slide
MBS154287 - A549 Cell Slide
Preparation and Storage
SIGIRR antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of SIGIRR expression in (A) A549 and (B) MCF7 cell lysate with SIGIRR antibody at 1 μg/ml.)
Western Blot (WB)
(Western blot analysis of SIGIRR expression in (A) A549 and (B) MCF7 cell lysate with SIGIRR antibody at 1 μg/ml.)
Immunocytochemistry (IHC)
(Immunocytochemistry of SIGIRR in A549 cells with SIGIRR antibody at 10 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of SIGIRR in A549 cells with SIGIRR antibody at 10 μg/mL.)
Related Product Information for anti-SIGIRR antibody
SIGIRR Antibody: SIGIRR is a member of the Toll-like receptor-interleukin 1 receptor superfamily. Members of this family are defined by the presence of an intracellular Toll-IL-1R (TIR) domain. The Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. SIGIRR was originally identified through database analysis and was shown to have only one Ig domain as opposed to the normal three Ig folds seen in the TIR family. Similar to ST2, another TIR family member, it has been shown to negatively regulate IL-1 receptor and Toll-like receptor signaling. However, SIGIRR inhibits TLR-IL-1R signaling by dimerizing with TLR4, TLR5, TLR9, and IL-1R. It also associates with the down-stream TLR signaling proteins IRAK and TRAF6 in an IL-1-dependent fashion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted MW: 45 kDa
Observed MW: 45 kDa
Observed MW: 45 kDa
NCBI Official Full Name
SIGIRR
NCBI Official Synonym Full Names
single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
NCBI Official Symbol
SIGIRR
NCBI Official Synonym Symbols
TIR8
NCBI Protein Information
single Ig IL-1-related receptor; toll/interleukin-1 receptor 8; single Ig IL-1R-related molecule; single immunoglobulin domain IL1R1 related; single immunoglobulin domain-containing IL1R-related protein
UniProt Protein Name
Single Ig IL-1-related receptor
Protein Family
UniProt Gene Name
SIGIRR
UniProt Synonym Gene Names
TIR8
UniProt Entry Name
SIGIR_HUMAN
Uniprot Description
SIGIRR: Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP. Belongs to the interleukin-1 receptor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: membrane; integral to membrane
Molecular Function: protein binding
Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of transcription factor activity; acute-phase response; negative regulation of cytokine and chemokine mediated signaling pathway; signal transduction; negative regulation of chemokine biosynthetic process
Research Articles on SIGIRR
Similar Products
Product Notes
The SIGIRR sigirr (Catalog #AAA150698) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIGIRR Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SIGIRR can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC). SIGIRR antibody can be used for the detection of SIGIRR by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the SIGIRR sigirr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGIRR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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