Western Blot (WB)
(Western blot analysis of extracts of THP-1 cells, using Phospho-TMEM173-S366 antibody at 1:1000 dilution.THP-1 cells were treated by PMA (80 nM) at 37℃ for Serum-starvation overnight.THP-1 cells were differentiation with TPA (200 nM) for 15 minutes and transfection with poly(dA:dT) (5 ug/mL) for 3 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Rabbit Phospho-TMEM173-S366 Polyclonal Antibody | anti-TMEM173-S366 antibody
Phospho-TMEM173-S366 Rabbit pAb
Gene Names
TMEM173; ERIS; MITA; MPYS; NET23; STING
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
Phospho-TMEM173-S366; Polyclonal Antibody; Phospho-TMEM173-S366 Rabbit pAb; TMEM173; ERIS; MITA; MPYS; NET23; SAVI; STING; hMITA; hSTING; anti-TMEM173-S366 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-TMEM173-S366 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A phospho specific peptide corresponding to residues surrounding S366 of human TMEM173.
Positive Samples
THP-1
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of THP-1 cells, using Phospho-TMEM173-S366 antibody at 1:1000 dilution.THP-1 cells were treated by PMA (80 nM) at 37℃ for Serum-starvation overnight.THP-1 cells were differentiation with TPA (200 nM) for 15 minutes and transfection with poly(dA:dT) (5 ug/mL) for 3 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Western Blot (WB)
(Western blot analysis of extracts of THP-1 cells, using Phospho-TMEM173-S366 antibody at 1:1000 dilution.THP-1 cells were treated by PMA (80 nM) at 37℃ for Serum-starvation overnight.THP-1 cells were differentiation with TPA (200 nM) for 15 minutes and transfection with poly(dA:dT) (5 ug/mL) for 3 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Related Product Information for anti-TMEM173-S366 antibody
Background: This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants.
Product Categories/Family for anti-TMEM173-S366 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
379
NCBI Official Full Name
stimulator of interferon genes protein
NCBI Official Synonym Full Names
transmembrane protein 173
NCBI Official Symbol
TMEM173
NCBI Official Synonym Symbols
ERIS; MITA; MPYS; NET23; STING
NCBI Protein Information
stimulator of interferon genes protein; hMITA; hSTING; mediator of IRF3 activation; endoplasmic reticulum IFN stimulator; mitochondrial mediator of IRF3 activation; endoplasmic reticulum interferon stimulator; N-terminal methionine-proline-tyrosine-serine
UniProt Protein Name
Stimulator of interferon genes protein
UniProt Gene Name
TMEM173
UniProt Synonym Gene Names
ERIS; MITA; STING; hSTING; ERIS; hMITA
UniProt Entry Name
STING_HUMAN
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Product Notes
The TMEM173-S366 tmem173 (Catalog #AAA9143914) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-TMEM173-S366 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-TMEM173-S366 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the TMEM173-S366 tmem173 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-TMEM173-S366, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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