Western Blot (WB)
(Anti-Cytokeratin 8 antibody, MBS175257, Western blottingLane 1: HELA Cell LysateLane 2: HT1080 Cell LysateLane 3: COLO320 Cell Lysate )
Rabbit Cytokeratin 8 Polyclonal Antibody | anti-KRT8 antibody
Anti-Cytokeratin 8 antibody
Western Blot (WB)
(Anti-Cytokeratin 8 antibody, MBS175257, Western blottingLane 1: HELA Cell LysateLane 2: HT1080 Cell LysateLane 3: COLO320 Cell Lysate )
Western Blot (WB)
(Anti-Cytokeratin 8 antibody, MBS175257, Western blottingLane 1: HELA Cell LysateLane 2: HT1080 Cell LysateLane 3: COLO320 Cell Lysate )
Immunohistochemistry (IHC)
(Anti-Cytokeratin 8 antibody, MBS175257, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-Cytokeratin 8 antibody, MBS175257, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Background: Cytokeratin 8(CK8), also known as Keratin 8(K8), is a type II keratin. Among the various intermediate filament(IF) proteins, cytokeratin 8(CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells. Cytokeratin 8 mRNA was abundant in fetal small intestine, placenta, pancreas, lung, liver, and kidney. Levels of cytokeratin 8 mRNA in placenta increased slightly during pregnancy. He et al.(2002) presented evidence that K8 is a new cytoplasmic target for JNK in Fas receptor-mediated signaling. The functional significance of this phosphorylation could relate to regulation of JNK signaling and/or regulation of keratin dynamics.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: dystrophin-associated glycoprotein complex; nucleoplasm; intermediate filament cytoskeleton; costamere; nuclear matrix; cytoplasm; keratin filament; intermediate filament; intercellular junction; Z disc; nucleus; sarcolemma
Molecular Function: protein binding; protein complex binding; structural molecule activity
Biological Process: tumor necrosis factor-mediated signaling pathway; viral reproduction; response to other organism; sarcomere organization; response to hydrostatic pressure
Disease: Cirrhosis, Familial
Research Articles on KRT8
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Product Notes
The KRT8 krt8 (Catalog #AAA175257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytokeratin 8 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the KRT8 krt8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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