Rabbit anti-Human PSMA4 Polyclonal Antibody | anti-PSMA4 antibody

Anti-PSMA4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104024

• Gene Names: PSMA4; HC9; PSC9; HsT17706
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: PSMA4; Polyclonal Antibody; Anti-PSMA4 Antibody; Rabbit Polyclonal; PSMA4 Antibody; Rabbit PAb; Antigen Affinity Purified; proteasome (prosome; macropain) subunit; alpha type; 4; Anti-HC9 Antibody; Anti-HsT17706 Antibody; Anti-PSC9 Antibody; anti-PSMA4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PSMA4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PSMA4 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Human PSMA4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PSMA4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PSMA4 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:29 kDa Observed band size:29 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PSMA4 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human PSMA4 in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-PSMA4 antibody

PSMA4 (Proteasome 20S Subunit Alpha 4) is a Protein Coding gene. The PSMA4 gene, located on 15q25.1, is conserved in chimpanzee, Rhesus monkey, cow, mouse, and rat, etc. This gene encodes a core alpha subunit of the 20S proteasome, which is a highly ordered ring-shaped structure composed of four rings of 28 non-identical subunits. PSMA4 is widely expressed in the appendix, lymph node, and other tissues. Diseases associated with PSMA4 include Cystic Fibrosis and Angelman Syndrome. Genome-wide association studies have linked the chromosome 15q25.1 locus to lung cancer susceptibility and implicated proteasome subunit alpha type-4 (PSMA4) as a candidate gene. The related pathways of PSMA4 are Regulation of activated PAK-2p34 by proteasome-mediated degradation and Antigen processing-Cross presentation.

References

Wang T, et al. (2015) Association of psma4 polymorphisms with lung cancer susceptibility and response to cisplatin-based chemotherapy in a chinese han population. Clin Transl Oncol 17 (7): 564-569.Takabe W, et al. (2000) Gene expression induced by bo-653, probucol and bhq in human endothelial cells. J Atheroscler Thromb 7 (4): 223-230.Shabek N, et al. (2009) Ubiquitin degradation with its substrate, or as a monomer in a ubiquitination-independent mode, provides clues to proteasome regulation. Proc Natl Acad Sci U S A 106 (29): 11907-11912.

NCBI and Uniprot Product Information

• NCBI GI #: 130861
• NCBI GeneID: 5685
• UniProt Accession #: P25789; Q53XP2; Q567Q5; Q8TBD1; D3DW86
• Molecular Weight: 261
• NCBI Official Full Name: Proteasome subunit alpha type-4
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, alpha type, 4
• NCBI Official Symbol: PSMA4
• NCBI Official Synonym Symbols: HC9; PSC9; HsT17706
• NCBI Protein Information: proteasome subunit alpha type-4; macropain subunit C9; proteasome subunit L; proteasome subunit HC9; proteasome component C9; multicatalytic endopeptidase complex subunit C9
• UniProt Protein Name: Proteasome subunit alpha type-4
• Protein Family: Proteasome
• UniProt Gene Name: PSMA4
• UniProt Synonym Gene Names: HC9; PSC9
• UniProt Entry Name: PSA4_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMA4: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Belongs to the peptidase T1A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.25.1; Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; intracellular membrane-bound organelle; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle