Rabbit GFR Alpha-3/GFRA3 Polyclonal Antibody | anti-GFRA3 antibody

Anti-GFR Alpha-3/GFRA3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103468

• Gene Names: Gfra3; Y15110; GFRalpha3
• Reactivity: Mouse, Rat; Predicted to React with: Human, Bovin (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: GFR Alpha-3/GFRA3; Polyclonal Antibody; Anti-GFR Alpha-3/GFRA3 Antibody; Rabbit Polyclonal; GFRA3 Antibody; Rabbit PAb; Antigen Affinity Purified; GDNF family receptor alpha 3; Anti-GFRalpha3 Antibody; Anti-Y15110 Antibody; anti-GFRA3 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat; Predicted to React with: Human, Bovin (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse GFR Alpha-3/GFRA3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GFRA3 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse GFRA3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse GFRA3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining GFRA3 in mouse heart with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining GFRA3 in mouse testis with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining GFRA3 in rat heart with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining GFRA3 in rat testis with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-GFRA3 antibody

Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 3 (GFRA3) or GDNFRa3 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA3/GDNFRa3 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA/GDNFRa acts as the ligand-binding component. The neurotrophic growth factor artemin binds selectively to GDNF family receptor alpha3 (GFRA3/GDNFRa3), forming a molecular complex with the co-receptor RET which mediates downstream signaling. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons.

References

Widenfalk J, et al. (2000) Neurturin, RET, GFRalpha-1 and GFRalpha-2, but not GFRalpha-3, mRNA are expressed in mice gonads. Cell Tissue Res. 299(3): 409-15.Li J, et al. (2009) Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice. FEBS Lett. 583(15): 2479-85.Yang C, et al. (2006) Distribution of GDNF family receptor alpha3 and RET in rat and human non-neural tissues. J Mol Histol. 37(1-2): 69-77.

NCBI and Uniprot Product Information

• NCBI GI #: 2627159
• NCBI GeneID: 14587
• UniProt Accession #: O35118; O35325; O55243; Q6NZC2; Q8C8L9
• Molecular Weight: 44,307 Da
• NCBI Official Full Name: Mus musculus mRNA for GFRalpha-3, complete cds
• NCBI Official Synonym Full Names: glial cell line derived neurotrophic factor family receptor alpha 3
• NCBI Official Symbol: Gfra3
• NCBI Official Synonym Symbols: Y15110; GFRalpha3
• NCBI Protein Information: GDNF family receptor alpha-3; GFR alpha-3; GFR-alpha-3; GDNFR-alpha-3; GDNF receptor alpha-3
• UniProt Protein Name: GDNF family receptor alpha-3
• Protein Family: GDNF family receptor
• UniProt Gene Name: Gfra3
• UniProt Synonym Gene Names: GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3
• UniProt Entry Name: GFRA3_MOUSE

NCBI Description

This gene encodes a cell surface glycoprotein and member of the glial cell line-derived neurotrophic receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein mediates binding of the ligand artemin to the ret receptor tyrosine kinase, and this interaction may regulate thermal pain and axon regeneration. Homozygous knockout mice for this gene exhibit impaired proliferation of cultured neuroblasts and impaired development of the superior cervical ganglion. [provided by RefSeq, Aug 2015]

Uniprot Description

GFRA3: Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; receptor activity; axon guidance receptor activity

Biological Process: axon guidance; sympathetic nervous system development; neuron migration; neuron development; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway