Rat Adenosine A2b Receptor ELISA Kit | ADORA2b elisa kit

Rat Adenosine A2b Receptor ELISA Kit

Cat. Num. AAA762150

• Gene Names: ADORA2B; ADORA2
• Reactivity: Rat
• Synonyms: Adenosine A2b Receptor; Rat Adenosine A2b Receptor ELISA Kit; ADORA2b; ADORA2b elisa kit

• Reactivity: Rat
• Sequence Length: 332

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ADORA2b elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat ADORA2b antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat ADORA2b antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat ADORA2b amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat ADORA2b can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 178150
• NCBI GeneID: 136
• Molecular Weight: 36,333 Da
• NCBI Official Full Name: adenosine A2b receptor
• NCBI Official Synonym Full Names: adenosine A2b receptor
• NCBI Official Symbol: ADORA2B
• NCBI Official Synonym Symbols: ADORA2
• NCBI Protein Information: adenosine receptor A2b
• UniProt Protein Name: Adenosine receptor A2b
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA2B
• UniProt Entry Name: AA2BR_HUMAN

NCBI Description

This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

ADORA2B: Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 17p12

Cellular Component: integral to plasma membrane; intracellular; plasma membrane

Molecular Function: adenosine receptor activity, G-protein coupled

Biological Process: activation of MAPK activity; adenosine receptor signaling pathway; adenylate cyclase activation; cellular defense response; cellular protein metabolic process; cellular response to extracellular stimulus; excretion; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; JNK cascade; positive regulation of chemokine production; positive regulation of chronic inflammatory response to non-antigenic stimulus; positive regulation of guanylate cyclase activity; positive regulation of interleukin-6 production; positive regulation of mast cell degranulation; relaxation of vascular smooth muscle