Typical Testing Data/Standard Curve (for reference only)
Human A2b/ADORA2B (Adenosine A2b Receptor) ELISA Kit | A2b/ADORA2B elisa kit
Human A2b/ADORA2B (Adenosine A2b Receptor) ELISA Kit
Gene Names
ADORA2B; ADORA2
Reactivity
Human
Synonyms
A2b/ADORA2B (Adenosine A2b Receptor); Human A2b/ADORA2B (Adenosine A2b Receptor) ELISA Kit; A2b/ADORA2B elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of A2b/ADORA2B. No significant cross-reactivity or interference between A2b/ADORA2B and analogues was observed.
Sequence Length
49
Samples
serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/ml
Sensitivity
<0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A2b/ADORA2B were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A2b/ADORA2B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for A2b/ADORA2B elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- A2b/ADORA2B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-A2b/ADORA2B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the A2b/ADORA2B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of A2b/ADORA2B can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36,333 Da
NCBI Official Full Name
adenosine A2b receptor, partial
NCBI Official Synonym Full Names
adenosine A2b receptor
NCBI Official Symbol
ADORA2B
NCBI Official Synonym Symbols
ADORA2
NCBI Protein Information
adenosine receptor A2b
UniProt Protein Name
Adenosine receptor A2b
UniProt Gene Name
ADORA2B
NCBI Description
This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Uniprot Description
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Research Articles on A2b/ADORA2B
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Product Notes
The Human A2b/ADORA2B adora2b (Catalog #AAA7607032) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7607032 ELISA Kit recognizes Human A2b/ADORA2B. It is sometimes possible for the material contained within the vial of "A2b/ADORA2B (Adenosine A2b Receptor), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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