Human FBXO32 (F-box only protein 32) ELISA Kit | FBXO32 elisa kit

Human FBXO32 (F-box only protein 32) ELISA Kit

Cat. Num. AAA7606617

• Gene Names: FBXO32; Fbx32; MAFbx
• Reactivity: Human
• Synonyms: FBXO32 (F-box only protein 32); Human FBXO32 (F-box only protein 32) ELISA Kit; FLJ32424; Fbx32; MAFbx; MGC33610; F-box only protein 32|atrogin 1|muscle atrophy F-box protein; FBXO32 elisa kit

• Reactivity: Human
• Sequence Length: 355

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 3.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Related Product Information for FBXO32 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FBXO32 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FBXO32 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FBXO32 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FBXO32 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20177894
• NCBI GeneID: 114907
• UniProt Accession #: Q969P5; A4KYM0
• Molecular Weight: 30,804 Da
• NCBI Official Full Name: F-box only protein 32
• NCBI Official Synonym Full Names: F-box protein 32
• NCBI Official Symbol: FBXO32
• NCBI Official Synonym Symbols: Fbx32; MAFbx
• NCBI Protein Information: F-box only protein 32
• UniProt Protein Name: F-box only protein 32
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO32
• UniProt Synonym Gene Names: MAFbx

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Uniprot Description

FBXO32: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: cytoplasm; cytosol; nucleoplasm; SCF ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: protein polyubiquitination; protein ubiquitination; response to denervation involved in regulation of muscle adaptation