Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Delphilin (GRID2IP) Standard Curve.)
Mouse Delphilin (GRID2IP) ELISA Kit | GRID2IP elisa kit
Mouse Delphilin (GRID2IP) ELISA Kit
Reactivity
Mouse
Synonyms
Delphilin (GRID2IP); Mouse Delphilin (GRID2IP) ELISA Kit; Delphilin; glutamate receptor; ionotropic; delta 2 (Grid2) interacting protein 1; GRID2IP elisa kit
Reactivity
Mouse
Specificity
Mouse Delphilin (GRID2IP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse GRID2IP. No significant cross-reactivity or interference between Mouse GRID2IP and analogues was observed.
Sequence Length
1203
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Delphilin (GRID2IP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Delphilin (GRID2IP) Standard Curve.)
Related Product Information for GRID2IP elisa kit
Description: This Mouse Delphilin (GRID2IP) ELISA Kit employs a two-site sandwich ELISA to quantitate GRID2IP in samples. An antibody specific for GRID2IP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRID2IP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRID2IP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRID2IP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Glutamate receptor delta-2 (GRID2) is predominantly expressed at parallel fiber-Purkinje cell postsynapses and plays crucial roles in synaptogenesis and synaptic plasticity. GRID2IP1 interacts with GRID2 and may control GRID2 signaling in Purkinje cells. The L-delphilin isoform contains an N-terminal PDZ domain (PDZ1), followed by a linker region, a second PDZ domain (PDZ2), an FH domain, and a C-terminal coiled-coil domain. Compared with L-delphilin, S-delphilin lacks PDZ1 and the linker region, which are replaced with an N-terminal palmitoylation site. Northern blot analysis of mouse brain showed higher expression of S-delphilin in cerebral cortex than cerebellum, whereas L-delphilin showed higher expression in cerebellum.
Background: Glutamate receptor delta-2 (GRID2) is predominantly expressed at parallel fiber-Purkinje cell postsynapses and plays crucial roles in synaptogenesis and synaptic plasticity. GRID2IP1 interacts with GRID2 and may control GRID2 signaling in Purkinje cells. The L-delphilin isoform contains an N-terminal PDZ domain (PDZ1), followed by a linker region, a second PDZ domain (PDZ2), an FH domain, and a C-terminal coiled-coil domain. Compared with L-delphilin, S-delphilin lacks PDZ1 and the linker region, which are replaced with an N-terminal palmitoylation site. Northern blot analysis of mouse brain showed higher expression of S-delphilin in cerebral cortex than cerebellum, whereas L-delphilin showed higher expression in cerebellum.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
delphilin isoform 1
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, delta 2 (Grid2) interacting protein 1
NCBI Official Symbol
Grid2ip
NCBI Protein Information
delphilin
UniProt Protein Name
Delphilin
Protein Family
UniProt Gene Name
Grid2ip
UniProt Entry Name
GRD2I_MOUSE
Uniprot Description
GRID2IP: Postsynaptic scaffolding protein at the parallel fiber- Purkinje cell synapse, where it may serve to link GRID2 with actin cytoskeleton and various signaling molecules.
Protein type: Adaptor/scaffold
Cellular Component: postsynaptic membrane; cell projection; membrane; plasma membrane; dendritic spine; synapse; cell junction
Molecular Function: protein binding
Biological Process: metabotropic glutamate receptor signaling pathway; actin cytoskeleton organization and biogenesis
Research Articles on GRID2IP
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Product Notes
The Mouse GRID2IP grid2ip (Catalog #AAA9718091) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718091 ELISA Kit recognizes Mouse GRID2IP. It is sometimes possible for the material contained within the vial of "Delphilin (GRID2IP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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