Rabbit anti-Human, Mouse DRD2 Polyclonal Antibody | anti-DRD2 antibody

DRD2, CT (DRD2, D(2) dopamine receptor, Dopamine D2 receptor) (Azide free) (HRP)

Cat. Num. AAA6293503

• Gene Names: DRD2; D2R; D2DR
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DRD2; Polyclonal Antibody; CT (DRD2; D(2) dopamine receptor; Dopamine D2 receptor) (Azide free) (HRP); Dopamine D2 receptor; anti-DRD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DRD2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DRD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-336 amino acids from the C-terminal region of human DRD2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DRD2 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DRD2 antibody detected the DRD2 protein (arrow).)

Related Product Information for anti-DRD2 antibody

This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing.

Product Categories/Family for anti-DRD2 antibody

Antibodies; Receptors

References

Verma, V., et al. J. Biol. Chem. 285(45):35092-35103(2010). Borroto-Escuela, D.O., et al. Biochem. Biophys. Res. Commun. 401(4):605-610(2010). Stelzel, C., et al. J. Neurosci. 30(42):14205-14212(2010). Huang, H.Y., et al. J. Formos. Med. Assoc. 109(10):736-739(2010). Itokawa, M., et al. J. Pharmacol. Sci. 114(1):1-5(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 17986270
• NCBI GeneID: 1813
• NCBI Accession #: NP_057658.2; NP_000786.1
• NCBI GenBank Nucleotide #: NM_016574.3
• UniProt Accession #: P14416; Q9NZR3; Q9UPA9
• Molecular Weight: 50,619 Da
• NCBI Official Full Name: D(2) dopamine receptor isoform short
• NCBI Official Synonym Full Names: dopamine receptor D2
• NCBI Official Symbol: DRD2
• NCBI Official Synonym Symbols: D2R; D2DR
• NCBI Protein Information: D(2) dopamine receptor; D(2) dopamine receptor; dopamine D2 receptor; dopamine receptor D2 isoform; seven transmembrane helix receptor
• UniProt Protein Name: D(2) dopamine receptor
• Protein Family: D(2) dopamine receptor
• UniProt Gene Name: DRD2
• UniProt Entry Name: DRD2_HUMAN

NCBI Description

This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD2: Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11); also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: synaptic vesicle membrane; axon; endocytic vesicle; integral to plasma membrane; postsynaptic density; dendrite; plasma membrane; acrosome; dendritic spine; perikaryon; nerve terminal; lateral plasma membrane

Molecular Function: dopamine D2 receptor-like receptor activity; ionotropic glutamate receptor binding; identical protein binding; protein binding; potassium channel regulator activity; protein homodimerization activity; protein heterodimerization activity; dopamine binding; drug binding

Biological Process: response to nicotine; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of dopamine uptake; prepulse inhibition; response to toxin; positive regulation of receptor internalization; thermoregulation; regulation of phosphoprotein phosphatase activity; positive regulation of multicellular organism growth; adult walking behavior; dopamine metabolic process; negative regulation of insulin secretion; dopamine receptor, adenylate cyclase inhibiting pathway; protein localization; negative regulation of blood pressure; phosphatidylinositol metabolic process; response to drug; response to light stimulus; response to inactivity; positive regulation of neuroblast proliferation; cerebral cortex GABAergic interneuron migration; negative regulation of synaptic transmission, glutamatergic; regulation of sodium ion transport; arachidonic acid secretion; regulation of synaptic transmission, GABAergic; positive regulation of growth hormone secretion; G-protein coupled receptor internalization; reduction of cytosolic calcium ion concentration; activation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; peristalsis; branching morphogenesis of a nerve; negative regulation of circadian sleep/wake cycle, sleep; response to morphine; locomotory behavior; behavioral response to ethanol; orbitofrontal cortex development; negative regulation of cell proliferation; synaptogenesis; behavioral response to cocaine; visual learning; adenohypophysis development; feeding behavior; nerve-nerve synaptic transmission; response to axon injury; circadian regulation of gene expression; negative regulation of dopamine secretion; negative regulation of cell migration; response to iron ion; associative learning; grooming behavior; positive regulation of cytokinesis; negative regulation of protein secretion; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; striatum development; regulation of heart rate; regulation of potassium ion transport; regulation of cAMP metabolic process; negative regulation of adenylate cyclase activity; sensory perception of smell; response to amphetamine; auditory behavior; regulation of dopamine uptake; response to cocaine; cellular calcium ion homeostasis; regulation of systemic arterial blood pressure by neurological process; negative regulation of dopamine receptor signaling pathway; pigmentation; axonogenesis; long-term memory; release of sequestered calcium ion into cytosol; positive regulation of G-protein coupled receptor protein signaling pathway; response to hypoxia; regulation of synapse structural plasticity; dopamine receptor, phospholipase C activating pathway

Disease: Myoclonic Dystonia