Human F-Box Protein 32 (FBXO32) ELISA Kit | FBXO32 elisa kit

Human F-Box Protein 32 (FBXO32) ELISA Kit

Cat. Num. AAA2022694

• Gene Names: FBXO32; Fbx32; MAFbx
• Reactivity: Human
• Synonyms: F-Box Protein 32 (FBXO32); Human F-Box Protein 32 (FBXO32) ELISA Kit; ATROGIN1; Fbx32; MAFbx; Muscle atrophy F-box protein; FBXO32 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FBXO32. No significant cross-reactivity or interference between FBXO32 and analogues was observed.
• Sequence Length: 262

• Samples: Human tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FBXO32 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FBXO32 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBXO32 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FBXO32. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FBXO32. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FBXO32, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FBXO32 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FBXO32 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 335057520
• NCBI GeneID: 114907
• NCBI Accession #: NP_001229392.1
• NCBI GenBank Nucleotide #: NM_001242463.1
• UniProt Accession #: Q969P5; A4KYM0
• Molecular Weight: 30,804 Da
• NCBI Official Full Name: F-box only protein 32 isoform 3
• NCBI Official Synonym Full Names: F-box protein 32
• NCBI Official Symbol: FBXO32
• NCBI Official Synonym Symbols: Fbx32; MAFbx
• NCBI Protein Information: F-box only protein 32
• UniProt Protein Name: F-box only protein 32
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO32
• UniProt Synonym Gene Names: MAFbx
• UniProt Entry Name: FBX32_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Uniprot Description

FBXO32: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8q24.13

Cellular Component: nucleolus; Z disc

Molecular Function: protein binding

Biological Process: protein ubiquitination; response to denervation involved in regulation of muscle adaptation