Rabbit ROCK1 Polyclonal Antibody | anti-ROCK1 antibody

ROCK1 Antibody

Cat. Num. AAA9613312

• Gene Names: ROCK1; ROCK-I; P160ROCK
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: ROCK1; Polyclonal Antibody; ROCK1 Antibody; coiled-coil-containing protein kinase 1; coiled-coil-containing protein kinase I; MGC131603; MGC43611; p160 Rhoassociated coiled coil-forming protein kinase; p160 ROCK-1; p160 ROCK1; p160ROCK; PRO0435; Renal carcinoma antigen NY REN 35; Renal carcinoma antigen NY-REN-35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho kinase; Rho-alpha kinase; Rho-associated; Rho-associated protein kinase 1; ROCK I; ROCK-I; ROCK1_HUMAN; Rok; rokalpha; anti-ROCK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ROCK1 Antibody detects endogenous levels of total ROCK1.; Tissue Specificity: Detected in blood platelets.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ROCK1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human ROCK1, corresponding to a region within the internal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Autophosphorylated on serine and threonine residues.Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
• Subunit Structure: Homodimer. Interacts with RHOB, RHOC, MYLC2B and PTEN. Interacts with ITGB1BP1 (via N-terminus and PTB domain) (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Interacts with SHROOM3.
• Similarity: The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
• Subcellular Location: Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Golgi apparatus membrane>Peripheral membrane protein. Cell projection>Bleb. Cytoplasm>Cytoskeleton. Cell membrane. Cell projection>Lamellipodium. Cell projection>Ruffle.; Note: Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of ROCK1 in lysates of NIH-3T3, using ROCK1 Antibody(AF4604).)

Related Product Information for anti-ROCK1 antibody

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity). Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 47605999
• NCBI GeneID: 6093
• UniProt Accession #: Q13464; Q2KHM4; Q59GZ4; B0YJ91
• Molecular Weight: 1354
• NCBI Official Full Name: Rho-associated protein kinase 1
• NCBI Official Synonym Full Names: Rho-associated, coiled-coil containing protein kinase 1
• NCBI Official Symbol: ROCK1
• NCBI Official Synonym Symbols: ROCK-I; P160ROCK
• NCBI Protein Information: rho-associated protein kinase 1; p160-ROCK; Rho kinase; p160 ROCK-1; renal carcinoma antigen NY-REN-35; rho-associated, coiled-coil-containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase I
• UniProt Protein Name: Rho-associated protein kinase 1
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: ROCK1
• UniProt Synonym Gene Names: ROCK-I; p160ROCK
• UniProt Entry Name: ROCK1_HUMAN

NCBI Description

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq, Jul 2008]

Uniprot Description

ROCK1: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN. Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Detected in blood platelets. Activated by RHOA binding. Inhibited by Y- 27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; AGC group; DMPK family; ROCK subfamily

Chromosomal Location of Human Ortholog: 18q11.1

Cellular Component: Golgi membrane; centriole; ruffle; cytoskeleton; lamellipodium; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; GTP-Rho binding; ATP binding; protein kinase activity

Biological Process: regulation of cell adhesion; axon guidance; apoptosis; bleb formation; signal transduction; protein amino acid phosphorylation; Rho protein signal transduction; negative regulation of angiogenesis; leukocyte adhesion; smooth muscle contraction; positive regulation of focal adhesion formation; regulation of stress fiber formation; regulation of actin cytoskeleton organization and biogenesis; membrane to membrane docking; myoblast migration; regulation of focal adhesion formation; ephrin receptor signaling pathway; negative regulation of neuron apoptosis; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; leukocyte tethering or rolling; regulation of keratinocyte differentiation; leukocyte migration; cell structure disassembly during apoptosis