Canine A Disintegrin And Metalloprotease 19 ELISA Kit | ADAM19 elisa kit

Canine A Disintegrin And Metalloprotease 19 ELISA Kit

Cat. Num. AAA753120

• Gene Names: ADAM19; MLTNB; FKSG34; MADDAM
• Reactivity: Canine
• Synonyms: A Disintegrin And Metalloprotease 19; Canine A Disintegrin And Metalloprotease 19 ELISA Kit; ADAM19 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ADAM19 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ADAM19. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM19 elisa kit

Principle of the assay: ADAM19 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM19 antibody and an ADAM19-HRP conjugate. The assay sample and buffer are incubated together with ADAM19-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM19 concentration since ADAM19 from samples and ADAM19-HRP conjugate compete for the anti-ADAM19 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM19 from the sample, fewer sites are left to bind ADAM19-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM19 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADAM19 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 38197238
• NCBI GeneID: 8728
• Molecular Weight: 100,848 Da
• NCBI Official Full Name: ADAM19 protein, partial
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 19
• NCBI Official Symbol: ADAM19
• NCBI Official Synonym Symbols: MLTNB; FKSG34; MADDAM
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 19; ADAM 19; meltrin-beta; metalloprotease-disintegrin meltrin beta; metalloprotease and disintegrin dendritic antigen marker; a disintegrin and metalloproteinase domain 19 (meltrin beta)
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 19
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM19
• UniProt Synonym Gene Names: MLTNB; FKSG34; ADAM 19; MADDAM
• UniProt Entry Name: ADA19_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has been demonstrated to be an active metalloproteinase, which may be involved in normal physiological processes such as cell migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. It is proposed to play a role in pathological processes, such as cancer, inflammatory diseases, renal diseases, and Alzheimer's disease. [provided by RefSeq, May 2013]

Uniprot Description

ADAM19: Participates in the proteolytic processing of beta-type neuregulin isoforms which are involved in neurogenesis and synaptogenesis, suggesting a regulatory role in glial cell. Also cleaves alpha-2 macroglobulin. May be involved in osteoblast differentiation and/or osteoblast activity in bone. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; SH3 domain binding

Biological Process: heart development; membrane protein ectodomain proteolysis