Human Adenylate cyclase type 10 ELISA Kit | ADCY10 elisa kit

Human Adenylate cyclase type 10 ELISA Kit

Cat. Num. AAA762039

• Gene Names: ADCY10; SAC; HCA2; SACI; Sacy; hsAC; HEL-S-7a
• Reactivity: Human
• Synonyms: Adenylate cyclase type 10; Human Adenylate cyclase type 10 ELISA Kit; ADCY10/Adenylate cyclase type 10/Testicular soluble adenylyl cyclase/Germ cell soluble adenylyl cyclase/hsAC/sAC/AH-related protein/A/denylate cyclase homolog; ADCY10 elisa kit

• Reactivity: Human
• Sequence Length: 1610

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ADCY10 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ADCY10 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ADCY10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADCY10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ADCY10 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 209976994
• NCBI GeneID: 55811
• NCBI Accession #: NP_060887.2
• NCBI GenBank Nucleotide #: NM_018417.5
• Molecular Weight: 169,750 Da
• NCBI Official Full Name: adenylate cyclase type 10 isoform 1
• NCBI Official Synonym Full Names: adenylate cyclase 10 (soluble)
• NCBI Official Symbol: ADCY10
• NCBI Official Synonym Symbols: SAC; HCA2; SACI; Sacy; hsAC; HEL-S-7a
• NCBI Protein Information: adenylate cyclase type 10
• UniProt Protein Name: Adenylate cyclase type 10
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY10
• UniProt Synonym Gene Names: SAC; hsAC; sAC
• UniProt Entry Name: ADCYA_HUMAN

NCBI Description

The protein encoded by this gene belongs to a distinct class of adenylyl cyclases that is soluble and insensitive to G protein or forskolin regulation. Activity of this protein is regulated by bicarbonate. Variation at this gene has been observed in patients with absorptive hypercalciuria. Alternatively spliced transcript variants encoding different isoforms have been observed. There is a pseudogene of this gene on chromosome 6. [provided by RefSeq, Jul 2014]

Uniprot Description

SAC: Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor. Involved in ciliary beat regulation. Genetic variations in ADCY10 are a cause of susceptibility to hypercalciuria absorptive type 2 (HCA2). Absorptive hypercalciuria is a common type of hypercalciuria, a condition characterized by excessive urinary calcium excretion. Absorptive hypercalciuria is due to gastrointestinal hyperabsorption of calcium and is a frequent cause of calcium oxalate nephrolithiasis. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; EC 4.6.1.1; Adenylyl cyclase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: apical part of cell; axon; cell soma; cytoplasm; cytosol; dendrite; growth cone; microtubule cytoskeleton; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: adenylate cyclase activity; ATP binding; ATPase binding; magnesium ion binding; manganese ion binding

Biological Process: cAMP biosynthetic process; positive regulation of apoptosis; spermatogenesis

Disease: Hypercalciuria, Absorptive, 2