Human Cysteine Rich Secretory Protein 3 (CRISP3) ELISA Kit | CRISP3 elisa kit

Human Cysteine Rich Secretory Protein 3 (CRISP3) ELISA Kit

Cat. Num. AAA2705770

• Gene Names: CRISP3; Aeg2; CRS3; SGP28; CRISP-3; dJ442L6.3
• Reactivity: Human
• Synonyms: Cysteine Rich Secretory Protein 3 (CRISP3); Human Cysteine Rich Secretory Protein 3 (CRISP3) ELISA Kit; CRS3; Aeg2; CRISP-3; SGP28; Specific granule protein of 28 kDa; CRISP3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Secretory Protein 3 (CRISP3).; No significant cross-reactivity or interference between Cysteine Rich Secretory Protein 3 (CRISP3) and analogues was observed.
• Sequence Length: 268

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Saliva, Sweat and Other Biological Fluids
• Detection Range: 0.94-60ng/mL
• Sensitivity: < 0.43ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Secretory Protein 3 (CRISP3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Secretory Protein 3 (CRISP3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRISP3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Secretory Protein 3 (CRISP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Secretory Protein 3 (CRISP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Secretory Protein 3 (CRISP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich Secretory Protein 3 (CRISP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 300244562
• NCBI GeneID: 10321
• NCBI Accession #: NP_001177915.1
• NCBI GenBank Nucleotide #: NM_001190986.2
• UniProt Accession #: P54108; Q15512; Q3MJ82; Q53FA9; Q5JW83; Q9H108; A8K9S1; B2R8I8
• Molecular Weight: 30,116 Da
• NCBI Official Full Name: cysteine-rich secretory protein 3 isoform 2
• NCBI Official Synonym Full Names: cysteine rich secretory protein 3
• NCBI Official Symbol: CRISP3
• NCBI Official Synonym Symbols: Aeg2; CRS3; SGP28; CRISP-3; dJ442L6.3
• NCBI Protein Information: cysteine-rich secretory protein 3
• UniProt Protein Name: Cysteine-rich secretory protein 3
• Protein Family: Cysteine-rich secretory protein
• UniProt Gene Name: CRISP3
• UniProt Synonym Gene Names: CRISP-3; SGP28

NCBI Description

This gene encodes a member of the cysteine-rich secretory protein (CRISP) family within the CRISP, antigen 5 and pathogenesis-related 1 proteins superfamily. The encoded protein has an N-terminal CRISP, antigen 5 and pathogenesis-related 1 proteins domain, a hinge region, and a C-terminal ion channel regulator domain. This protein contains cysteine residues, located in both the N- and C-terminal domains, that form eight disulfide bonds, a distinguishing characteristic of this family. This gene is expressed in the male reproductive tract where it plays a role in sperm function and fertilization, and the female reproductive tract where it plays a role in endometrial receptivity for embryo implantation. This gene is upregulated in certain types of prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]