Human Growth Differentiation Factor 11 ELISA Kit | GDF11 elisa kit

Human Growth Differentiation Factor 11 ELISA Kit

Cat. Num. AAA730218

• Gene Names: GDF11; BMP11; BMP-11
• Reactivity: Human
• Synonyms: Growth Differentiation Factor 11; Human Growth Differentiation Factor 11 ELISA Kit; GDF11 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This GDF-11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GDF-11. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDF11 elisa kit

Principle of the assay: GDF-11 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GDF-11. Standards or samples are then added to the microtiter plate wells and GDF-11 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GDF-11 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GDF-11 are added to each well to "sandwich" the GDF-11 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GDF-11 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF-11 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GDF11 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 5031613
• NCBI GeneID: 10220
• NCBI Accession #: NP_005802.1
• NCBI GenBank Nucleotide #: NM_005811.3
• Molecular Weight: 45,091 Da
• NCBI Official Full Name: growth/differentiation factor 11
• NCBI Official Synonym Full Names: growth differentiation factor 11
• NCBI Official Symbol: GDF11
• NCBI Official Synonym Symbols: BMP11; BMP-11
• NCBI Protein Information: growth/differentiation factor 11; GDF-11; bone morphogenetic protein 11
• UniProt Protein Name: Growth/differentiation factor 11
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF11
• UniProt Synonym Gene Names: BMP11; GDF-11; BMP-11
• UniProt Entry Name: GDF11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF11: Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: nervous system development; cell maturation; palate development; regulation of apoptosis; negative regulation of cell proliferation; negative regulation of cell differentiation; camera-type eye morphogenesis; spinal cord anterior/posterior patterning; ureteric bud development; regulation of MAPKKK cascade; pancreas development; mesoderm development; cell development; skeletal development; metanephros development; growth