Canine Growth Differentiation Factor 11 ELISA Kit | GDF11 elisa kit

Canine Growth Differentiation Factor 11 ELISA Kit

Cat. Num. AAA738532

• Gene Names: GDF11; BMP11; BMP-11
• Reactivity: Canine
• Synonyms: Growth Differentiation Factor 11; Canine Growth Differentiation Factor 11 ELISA Kit; GDF11 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This GDF-11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GDF-11. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDF11 elisa kit

Principle of the assay: GDF-11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDF-11 antibody and an GDF-11-HRP conjugate. The assay sample and buffer are incubated together with GDF-11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF-11 concentration since GDF-11 from samples and GDF-11-HRP conjugate compete for the anti-GDF-11 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF-11 from the sample, fewer sites are left to bind GDF-11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF-11 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GDF11 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 5031613
• NCBI GeneID: 10220
• NCBI Accession #: NP_005802.1
• NCBI GenBank Nucleotide #: NM_005811.3
• Molecular Weight: 45,091 Da
• NCBI Official Full Name: growth/differentiation factor 11
• NCBI Official Synonym Full Names: growth differentiation factor 11
• NCBI Official Symbol: GDF11
• NCBI Official Synonym Symbols: BMP11; BMP-11
• NCBI Protein Information: growth/differentiation factor 11; GDF-11; bone morphogenetic protein 11
• UniProt Protein Name: Growth/differentiation factor 11
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF11
• UniProt Synonym Gene Names: BMP11; GDF-11; BMP-11
• UniProt Entry Name: GDF11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF11: Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; nervous system development; negative regulation of cell proliferation; negative regulation of cell differentiation; camera-type eye morphogenesis; spinal cord anterior/posterior patterning; ureteric bud development; regulation of MAPKKK cascade; pancreas development; cell maturation; mesoderm development; palate development; cell development; metanephros development; skeletal development; growth