Human Matrix metalloproteinase 8/Neutrophil collagenase, MMP-8 ELISA Kit | MMP-8 elisa kit

Human Matrix metalloproteinase 8/Neutrophil collagenase, MMP-8 ELISA Kit

Cat. Num. AAA162248

• Gene Names: MMP8; HNC; CLG1; MMP-8; PMNL-CL
• Reactivity: Human
• Synonyms: Matrix metalloproteinase 8/Neutrophil collagenase; MMP-8; Human Matrix metalloproteinase 8/Neutrophil collagenase; MMP-8 ELISA Kit; MMP-8 elisa kit

• Reactivity: Human
• Sequence Length: 467

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml - 10ng/ml
• Sensitivity: 0.021ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP-8 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Matrix Metalloproteinase 8/Neutrophil Collagenase (also known as MMP-8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human MMP-8 antibody. MMP-8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human MMP-8 Antibody is added and binds to MMP-8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MMP-8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human MMP-8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 71648770
• NCBI GeneID: 4317
• UniProt Accession #: P22894; Q45F99
• Molecular Weight: 53,412 Da
• NCBI Official Full Name: matrix metalloproteinase 8 (neutrophil collagenase)
• NCBI Official Synonym Full Names: matrix metallopeptidase 8
• NCBI Official Symbol: MMP8
• NCBI Official Synonym Symbols: HNC; CLG1; MMP-8; PMNL-CL
• NCBI Protein Information: neutrophil collagenase
• UniProt Protein Name: Neutrophil collagenase
• UniProt Gene Name: MMP8
• UniProt Synonym Gene Names: CLG1; MMP-8; PMNL-CL

NCBI Description

This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

Can degrade fibrillar type I, II, and III collagens.