Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver tissue using MBS7048548 at dilution of 1:100)
Rabbit anti-Human KRT7 Polyclonal Antibody | anti-KRT7 antibody
KRT7 Antibody
IF: 1: 50-1: 200
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver tissue using MBS7048548 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver tissue using MBS7048548 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human cervical cancer using MBS7048548 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human cervical cancer using MBS7048548 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunofluorescent analysis of HepG2 cells using MBS7048548 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC)
(Immunofluorescent analysis of HepG2 cells using MBS7048548 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate,HepG2 whole cell lysate,A549 whole cell lysateAll lanes: KRT7 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52 KDaObserved band size: 52 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate,HepG2 whole cell lysate,A549 whole cell lysateAll lanes: KRT7 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52 KDaObserved band size: 52 KDa)
Immunoprecipitation (IP)
(Immunoprecipitating KRT7 in HepG2 whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of MBS7048548 in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7048548 (8ug)+ HepG2 whole cell lysate (500ug)Lane 3: HepG2 whole cell lysate (10ug))
Immunoprecipitation (IP)
(Immunoprecipitating KRT7 in HepG2 whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of MBS7048548 in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7048548 (8ug)+ HepG2 whole cell lysate (500ug)Lane 3: HepG2 whole cell lysate (10ug))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
Uniprot Description
K7: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding
Research Articles on KRT7
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Product Notes
The KRT7 krt7 (Catalog #AAA7048548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT7 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 1: 20-1: 200 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the KRT7 krt7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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