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Western Blot (WB) (Western blot analysis of Cytokeratin 7 expression in HeLa cell lysate.)

Rabbit Cytokeratin 7 Polyclonal Antibody | anti-CK-7 antibody

Anti-Cytokeratin 7 Rabbit pAb

Gene Names
KRT7; K7; CK7; SCL; K2C7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Affinity Chromatography
Synonyms
Cytokeratin 7; Polyclonal Antibody; Anti-Cytokeratin 7 Rabbit pAb; Cytokeratin-7; Keratin 7; Keratin7; Keratin-7; KRT7; SCL; Sarcolectin; Type-II keratin Kb7; Krt2-7; Keratin type II cytoskeletal 7; anti-CK-7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Cytokeratin 7 Antibody detects endogenous levels of Cytokeratin 7
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
469
Applicable Applications for anti-CK-7 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:500
ICC/IF: 1:50-1:200
Immunogen
A synthesized peptide derived from human Cytokeratin 7
Subcellular Location
Secreted; Extracellular space; Extracellular matrix
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of Cytokeratin 7 expression in HeLa cell lysate.)

Western Blot (WB) (Western blot analysis of Cytokeratin 7 expression in HeLa cell lysate.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human uterus, using Cytokeratin 7 Antibody.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human uterus, using Cytokeratin 7 Antibody.)

Immunofluorescence (IF)

(Immunofluorescent analysis of HeLa cells, using Cytokeratin 7 Antibody.)

Immunofluorescence (IF) (Immunofluorescent analysis of HeLa cells, using Cytokeratin 7 Antibody.)
Related Product Information for anti-CK-7 antibody
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51kDa
NCBI Official Full Name
keratin, type II cytoskeletal 7
NCBI Official Synonym Full Names
keratin 7
NCBI Official Symbol
KRT7
NCBI Official Synonym Symbols
K7; CK7; SCL; K2C7
NCBI Protein Information
keratin, type II cytoskeletal 7
UniProt Protein Name
Keratin, type II cytoskeletal 7
UniProt Gene Name
KRT7
UniProt Synonym Gene Names
SCL; CK-7; K7

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

Research Articles on CK-7

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Product Notes

The CK-7 krt7 (Catalog #AAA4750510) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytokeratin 7 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:100-1:500 ICC/IF: 1:50-1:200. Researchers should empirically determine the suitability of the CK-7 krt7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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