Typical Testing Data/Standard Curve (for reference only)
Rat chemokine (C-X-C motif) receptor 4 ELISA Kit | CXCR4 elisa kit
Rat CXC-chemokine receptor 4, CXCR4 ELISA Kit
Reactivity
Rat
Synonyms
chemokine (C-X-C motif) receptor 4; Rat CXC-chemokine receptor 4; CXCR4 ELISA Kit; Rat CXC-chemokine receptor 4 (CXCR4) ELISA Kit; CD184; D2S201E; FB22; HM89; HSY3RR; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; C-X-C chemokine receptor type 4; CD184 antigen; fusin; leukocyte-derived seven-transmembrane-domain receptor; lip; CXCR4 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat CXCR4. No significant cross-reactivity or interference between rat CXCR4 and analogues was observed.
Sequence Length
349
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Sandwich
Detection Range
0.156 ng/ml -10 ng/ml.
Sensitivity
The minimum detectable dose of rat CXCR4 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CXCR4 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CXCR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCR4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
39,334 Da
NCBI Official Full Name
C-X-C chemokine receptor type 4
NCBI Official Synonym Full Names
chemokine (C-X-C motif) receptor 4
NCBI Official Symbol
Cxcr4
NCBI Protein Information
C-X-C chemokine receptor type 4; LESTR; fusin; CXC-R4; CXCR-4; SDF-1 receptor; CXC chemokine receptor; chemokine receptor (LCR1); stromal cell-derived factor 1 receptor; leukocyte-derived seven transmembrane domain receptor
UniProt Protein Name
C-X-C chemokine receptor type 4
Protein Family
UniProt Gene Name
Cxcr4
UniProt Synonym Gene Names
Cmkar4; CXC-R4; CXCR-4; LESTR; SDF-1 receptor
UniProt Entry Name
CXCR4_RAT
NCBI Description
G protein-coupled receptor for the chemokine stromal cell-derived factor-1; may have a role in brain development [RGD, Feb 2006]
Uniprot Description
Function: Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes
Research Articles on CXCR4
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Product Notes
The Rat CXCR4 cxcr4 (Catalog #AAA701239) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701239 ELISA Kit recognizes Rat CXCR4. It is sometimes possible for the material contained within the vial of "chemokine (C-X-C motif) receptor 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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