Human Chemokine C-X-C-Motif Receptor 4 (CXCR4) ELISA Kit | CXCR4 elisa kit

Human Chemokine C-X-C-Motif Receptor 4 (CXCR4) ELISA Kit

Cat. Num. AAA454216

• Gene Names: CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; D2S201E
• Reactivity: Human
• Synonyms: Chemokine C-X-C-Motif Receptor 4 (CXCR4); Human Chemokine C-X-C-Motif Receptor 4 (CXCR4) ELISA Kit; CD184 Fusin NPYR HSY3RR LAP3 LCR1 LESTR NPY3R NPYRL WHIM Leukocyte-derived seven transmembrane domain receptor Lipopolysaccharide-associated protein 3; CXCR4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of QCXCR4. No significant cross-reactivity or interference between CXCR4 and analogues was observed.
• Sequence Length: 356

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.060ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CXCR4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CXCR4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CXCR4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CXCR4 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CXCR4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CXCR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CXCR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CXCR4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 56790927
• NCBI GeneID: 7852
• NCBI Accession #: NP_001008540.1
• NCBI GenBank Nucleotide #: NM_001008540.2
• UniProt Accession #: P61073; O60835; P30991; P56438; Q53S69; Q9BXA0; Q9UKN2; B2R5N0
• Molecular Weight: 40,221 Da
• NCBI Official Full Name: C-X-C chemokine receptor type 4 isoform a
• NCBI Official Synonym Full Names: C-X-C motif chemokine receptor 4
• NCBI Official Symbol: CXCR4
• NCBI Official Synonym Symbols: FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; D2S201E
• NCBI Protein Information: C-X-C chemokine receptor type 4
• UniProt Protein Name: C-X-C chemokine receptor type 4
• Protein Family: C-X-C chemokine receptor
• UniProt Gene Name: CXCR4
• UniProt Synonym Gene Names: CXC-R4; CXCR-4; LESTR; LAP-3; LPS-associated protein 3; SDF-1 receptor

NCBI Description

This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival.