Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) ELISA Kit | GOT1 elisa kit

Human Aspartate aminotransferase, AST ELISA Kit

Cat. Num. AAA700831

• Gene Names: GOT1; cCAT; GIG18; cAspAT; ASTQTL1
• Reactivity: Human
• Synonyms: glutamic-oxaloacetic transaminase 1; soluble (aspartate aminotransferase 1); Human Aspartate aminotransferase; AST ELISA Kit; GIG18; aspartate aminotransferase 1; growth-inhibiting protein 18; GOT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GOT1. No significant cross-reactivity or interference between Human GOT1 and analogues was observed.
• Sequence Length: 413

• Samples: Serum, urine
• Detection Range: 3.12 mIU/ml-200 mIU/ml
• Sensitivity: 0.78 mIU/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GOT1 elisa kit

Introduction: Aspartate transaminase (AST) also called serum glutamic oxaloacetic transaminase (SGOT) or aspartate aminotransferase (ASAT/AAT) is similar to alanine transaminase (ALT) in that it is another enzyme associated with liver parenchymal cells. It is raised in acute liver damage. It is also present in red blood cells and cardiac muscle, skeletal muscle, and kidney and brain tissue, and may be elevated due to damage to those sources as well. AST was defined as a biochemical marker for the identification of acute myocardial infarction in 1954. However the use of AST for such a identification is now redundant and has been superseded by the cardiac troponins. AST (SGOT) is commonly measured clinically as a part of liver function tests, to determine liver health.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AST. Standards or samples are then added to the appropriate microtiter plate wells with a 3 biotin-conjugated antibody preparation specific for AST and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504067
• NCBI GeneID: 2805
• NCBI Accession #: NP_002070.1
• NCBI GenBank Nucleotide #: NM_002079.2
• UniProt Accession #: P17174; Q5VW80; B2R6R7; B7Z7E9
• Molecular Weight: 46,248 Da
• NCBI Official Full Name: aspartate aminotransferase, cytoplasmic
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 1, soluble
• NCBI Official Symbol: GOT1
• NCBI Official Synonym Symbols: cCAT; GIG18; cAspAT; ASTQTL1
• NCBI Protein Information: aspartate aminotransferase, cytoplasmic; transaminase A; aspartate transaminase 1; aspartate aminotransferase 1; growth-inhibiting protein 18; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; cysteine aminotransferase, cytoplasmic
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Protein transport protein
• UniProt Gene Name: GOT1
• UniProt Synonym Gene Names: cAspAT; cCAT
• UniProt Entry Name: AATC_HUMAN

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.1 PublicationManual assertion based on experiment in:Ref.9

Catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.

Cofactor: Pyridoxal phosphate.