Aspartate Aminotransferase Recombinant Protein | GOT1 recombinant protein

Aspartate Aminotransferase Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121638

• Gene Names: GOT1; cCAT; GIG18; cAspAT; ASTQTL1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Aspartate Aminotransferase; Aspartate Aminotransferase Protein; Human; Recombinant (His Tag); Human GOT1/Aspartate aminotransferase Protein (His Tag); ASTQTL1 Protein; cAspAT Protein; cCAT Protein; GIG18 Protein; glutamic-oxaloacetic transaminase 1; soluble; GOT1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln413

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human GOT1 (P17174)(Met1-Gln413) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GOT1 recombinant protein

Background: GOT1 (Glutamic-Oxaloacetic Transaminase 1) is a Protein Coding gene. GOT1 belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme that exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. GOT1 is an important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. GOT1 is broadly expressed in the heart, liver, and other tissues. Diseases associated with GOT1 include Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1, and Lujo Hemorrhagic Fever.

References

Shen H, et al. (2011) Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels. J Hum Genet. 56(11):801-5. Doonan S, et al. (1985) Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int J Biochem. 16(12):1193-9. Panteghini M. (1990) Aspartate aminotransferase isoenzymes. Clin Biochem. 23(4):311-9. Bousquet-Lemercier B, et al. (1990) Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection. Biochemistry. 29(22):5293-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4504067
• NCBI GeneID: 2805
• NCBI Accession #: NP_002070.1
• NCBI GenBank Nucleotide #: NM_002079.2
• UniProt Accession #: P17174; Q5VW80; B2R6R7; B7Z7E9
• Molecular Weight: 44,147 Da
• NCBI Official Full Name: aspartate aminotransferase, cytoplasmic
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 1, soluble
• NCBI Official Symbol: GOT1
• NCBI Official Synonym Symbols: cCAT; GIG18; cAspAT; ASTQTL1
• NCBI Protein Information: aspartate aminotransferase, cytoplasmic; aspartate aminotransferase 1; aspartate transaminase 1; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; growth-inhibiting protein 18; transaminase
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Aspartate aminotransferase
• UniProt Gene Name: GOT1
• UniProt Synonym Gene Names: cAspAT; cCAT
• UniProt Entry Name: AATC_HUMAN

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

Uniprot Description

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - cysteine and methionine; Transferase; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.3

Chromosomal Location of Human Ortholog: 10q24.1-q25.1

Cellular Component: lysosome; cytoplasm; nerve terminal; nucleus; cytosol

Molecular Function: cysteine transaminase activity; phosphatidylserine decarboxylase activity; carboxylic acid binding; aspartate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; oxaloacetate metabolic process; glutamate catabolic process to aspartate; response to glucocorticoid stimulus; glucose metabolic process; aspartate biosynthetic process; pathogenesis; gluconeogenesis; 2-oxoglutarate metabolic process; methionine salvage; cellular response to insulin stimulus; sulfur amino acid metabolic process; glycerol biosynthetic process; fatty acid homeostasis; carbohydrate metabolic process; aspartate catabolic process; aspartate metabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate; polyamine metabolic process

Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1