Neurturin Active Protein | NRTN active protein

Human Neurturin

Cat. Num. AAA692075

• Gene Names: NRTN; NTN
• Reactivity: Human
• Purity: > 98% by SDS-PAGE and HPLC analysis
• Synonyms: Neurturin; Human Neurturin; Recombinant Human Neurturin; NRTN active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 98% by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized from 10 mM Sodium Citrate, pH 4.0
• Sequence: ARLGARPCGL RELEVRVSEL GLGYASDETV LFRYCAGACE AAARVY DLGL RRLRQRRRLR RERVRAQPCC RPTAYEDEVS FLDAHSRYHT V HELSARECA CV
• Sequence Length: 197

• Structural Information: disulphide-linked homodimer
• Endotoxin Level: < 0.1 ng/ug per ug of protein
• Biological Activity: Human Neurturin at a concentration of 100 ng/ml will support the survival of 65% of newborn rat sympathetic neurons.
• Length (aa): 204
• Stability: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2°C to 8°C gor up to 1 week. For extended storage , it is recommended to further dilute in a buffer containing a carrier protien (example 0.1% BSA) and store in working aliquots at -20C to -80°C.
• Preparation and Storage: Store at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C

Related Product Information for NRTN active protein

Neurturin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, Artemin, and Persephin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Neurturin signals through a multicomponent receptor system, composed of RET and one of four GFR alpha (alpha1-alpha4) receptors. Neurturin promotes the development and survival of sympathetic and sensory neurons by signaling through a receptor system composed of RET and GFRalpha2. The functional form of human Neurturin is a disulfide-linked homodimer, of two 11.8 kDa polypeptide monomers (204 total amino acid residues). Each monomer contains seven conserved cysteine residues, one of which (Cys 69) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

Product Categories/Family for NRTN active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4758826
• NCBI GeneID: 4902
• NCBI Accession #: NP_004549.1
• NCBI GenBank Nucleotide #: NM_004558.3
• UniProt Accession #: Q99748; B2RPE8
• Molecular Weight: 11.8 kDa
• NCBI Official Full Name: neurturin preproprotein
• NCBI Official Synonym Full Names: neurturin
• NCBI Official Symbol: NRTN
• NCBI Official Synonym Symbols: NTN
• NCBI Protein Information: neurturin; prepro-neurturin
• UniProt Protein Name: Neurturin
• Protein Family: Neurturin
• UniProt Gene Name: NRTN
• UniProt Entry Name: NRTN_HUMAN

NCBI Description

Neurturin is a member of the TGF-beta subfamily, TRN. This gene signals through RET and a GPI-linked coreceptor, and promotes survival of neuronal populations. A neurturin mutation has been described in a family with Hirschsprung Disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.

Subunit structure: Homodimer; disulfide-linked.

Subcellular location: Secreted.

Involvement in disease: Genetic variations in NRTN may contribute to Hirschsprung disease, in association with mutations of RET gene, and possibly mutations in other loci. Hirschsprung disease is a disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Ref.5

Sequence similarities: Belongs to the TGF-beta family. GDNF subfamily.