Rabbit FANCA Polyclonal Antibody | anti-FANCA antibody

FANCA (Phospho-S1149) Polyclonal Antibody

Cat. Num. AAA3005173

• Gene Names: FANCA; FA; FA1; FAA; FAH; FA-H; FACA; FANCH
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FANCA; Polyclonal Antibody; FANCA (Phospho-S1149) Polyclonal Antibody; Fanconi anemia group A protein; Protein FACA; FAA; FACA; FANCH; anti-FANCA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-FANCA (S1149) polyclonal antibody detects endogenous levels of FANCA protein only when phosphorylated at Ser1149.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1455

• Applicable Applications for anti-FANCA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; ICC: 1:50-1:200; IP: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human FANCA around the phosphorylation site of Serine 1149.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-FANCA (S1149) polyclonal antibody at 1:500 dilutionLane1:HepG2 cell lysate treated with EGF (0.1ng/ML,30mins) Lane2:sp2/0 cell lysate treated with EGF (0.1ng/ML,30mins) Lane3:H9C2 cell lysate treated with EGF (0.1ng/ML,30mins))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-FANCA (S1149) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-FANCA antibody

Fanconi anemia (FA) is an autosomal recessive disorder characterized by bone marrow failure, birth defects and chromosomal instability. At the cellular level, FA is characterized by spontaneous chromosomal breakage and a unique hypersensitivity to DNA cross-linking agents. At least eight complementation groups (A-G) have been identified and six FA genes (for subtypes A, C, D2, E, F and G) have been cloned. The FA proteins lack sequence homologies or motifs that could point to a molecular function. The cellular accumulation of FA proteins, including FANCA and FANCG, is subject to regulation by TNFalpha signaling. Phosphorylation of FANC (Fanconi anemia complementation group) proteins is thought to be important for the function of the FA pathway. FANCA, also known as FACA and FANCH, associates with the Brm-related gene 1 (BRG1) product, a subunit of the SWI/SNF complex which remodels chromatin structure through a DNA-dependent ATPase activity. FANCA is mainly expressed in lymphoid tissues, testis and ovary. The amino-terminal region of the FANCA protein is required for FANCG binding, FANCC binding, nuclear localization and functional activity of the complex.

NCBI and Uniprot Product Information

• NCBI GI #: 66880553
• NCBI GeneID: 2175
• NCBI Accession #: NP_000126.2
• NCBI GenBank Nucleotide #: NP_000126.2
• UniProt Accession #: O15360
• Molecular Weight: ~ 163kDa
• NCBI Official Full Name: Fanconi anemia group A protein isoform a
• NCBI Official Synonym Full Names: FA complementation group A
• NCBI Official Symbol: FANCA
• NCBI Official Synonym Symbols: FA; FA1; FAA; FAH; FA-H; FACA; FANCH
• NCBI Protein Information: Fanconi anemia group A protein
• UniProt Protein Name: Fanconi anemia group A protein
• Protein Family: Fanconi anemia group A protein
• UniProt Gene Name: FANCA
• UniProt Synonym Gene Names: FAA; FACA; FANCH; Protein FACA
• UniProt Entry Name: FANCA_HUMAN

NCBI Description

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are the most common cause of Fanconi anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

FANCA: DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Defects in FANCA are a cause of Fanconi anemia (FA). FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: male gonad development; protein complex assembly; male meiosis; DNA repair; female gonad development; regulation of cell proliferation

Disease: Fanconi Anemia, Complementation Group A; Tracheoesophageal Fistula With Or Without Esophageal Atresia