Rabbit anti-Human Neurturin Polyclonal Antibody | anti-NRTN antibody

Neurturin Antibody

Cat. Num. AAA151317

• Gene Names: NRTN; NTN
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Neurturin Antibody is affinity chromatography purified via peptide column.
• Synonyms: Neurturin; Polyclonal Antibody; Neurturin Antibody; NTN; neurturin; anti-NRTN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Neurturin Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 197

• Applicable Applications for anti-NRTN antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Neurturin antibody can be used for detection of neurturin by Western blot. An approximate 14 kDa band of the full length recombinant NTN was detected. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Neurturin antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Neurturin.
• Buffer: Neurturin Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Neurturin antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NTN in HeLa cell lyaste containing 10 or 2.5 ng of full length recombinant NTN with neurturin antibody at 1:500.)

Immunohistochemistry (IHC)

(Immunohistochemistry of neurturin in human brain tissue with neurturin antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.Green: Neuriturin Antibody (1121)Red: Phylloidin stainingBlue: DAPI staining)

Immunohistochemistry (IHC)

(Immunohistochemistry of Neurturin in human brain tissue with Neurturin antibody at 5 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Neurturin in mouse brain tissue with Neurturin Antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Neurturin in mouse brain tissue with Neurturin Antibody at 20 μg/mL.Green: Neuriturin Antibody (1121)Red: Phylloidin stainingBlue: DAPI staining)

Related Product Information for anti-NRTN antibody

Neurturin Antibody: Glial cell line-derived neurotrophic factor (GDNF) plays key roles in the control of vertebrate neuron survival and differentiation. A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin. Physiological responses to neurturin (NTN) require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRalpha. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently and termed GDNFRβ, Ret ligand 2 (RETL2) or TGF-beta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. NTN binds to and forms a complex with GFRα-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway. Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations. Neurturin and GDNF define a new family of neurotrophic factors.

NCBI and Uniprot Product Information

• NCBI GI #: 4758826
• NCBI GeneID: 4902
• NCBI Accession #: NP_004549
• NCBI GenBank Nucleotide #: NM_004558.3
• UniProt Accession #: Q99748; B2RPE8
• Molecular Weight: 14 kDa
• NCBI Official Full Name: neurturin preproprotein
• NCBI Official Synonym Full Names: neurturin
• NCBI Official Symbol: NRTN
• NCBI Official Synonym Symbols: NTN
• NCBI Protein Information: neurturin; prepro-neurturin
• UniProt Protein Name: Neurturin
• Protein Family: Neurturin
• UniProt Gene Name: NRTN
• UniProt Entry Name: NRTN_HUMAN

NCBI Description

Neurturin is a member of the TGF-beta subfamily, TRN. This gene signals through RET and a GPI-linked coreceptor, and promotes survival of neuronal populations. A neurturin mutation has been described in a family with Hirschsprung Disease. [provided by RefSeq, Jul 2008]

Uniprot Description

NRTN: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. Genetic variations in NRTN may contribute to Hirschsprung disease, in association with mutations of RET gene, and possibly mutations in other loci. Hirschsprung disease is a disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Belongs to the TGF-beta family. GDNF subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: axon; extracellular region

Molecular Function: growth factor activity; receptor binding

Biological Process: axon guidance; nervous system development; MAPKKK cascade; nerve development; neural crest cell migration; transmembrane receptor protein tyrosine kinase signaling pathway; neurite development

Disease: Hirschsprung Disease, Susceptibility To, 1