Klotho Active Protein | KL active protein

Human Klotho

Cat. Num. AAA691589

• Reactivity: Human
• Purity: 98 % (SDS-PAGE, HPLC)
• Synonyms: Klotho; Human Klotho; Recombinant Human Klotho; KL active protein

• Host: CHO Cells
• Reactivity: Human
• Purity/Purification: 98 % (SDS-PAGE, HPLC)
• Form/Format: Lyophilized
• Sequence: EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGG WQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD V ASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGL RYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DH FRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSP RL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPR RMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDF T ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQL LSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKML LPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH
• Sequence Length: 1012

• Endotoxin Level: < 0.1 ng per ug of IL-33
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of murine NIH-3T3 cells. Recombinant human Klotho is effective in a concentration range of 0.5-2.0 ug/ml.

Related Product Information for KL active protein

Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon,. The full length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively, The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128 kDa has been identified in the blood and cerebrospinal fluid. This circulating protein arises from the action of an as yet unidentified protease which cleaves transmembrane Klotho just above and/or within the plasma membrane. Klotho has been shown to play a key role in the signaling cascade of fibroblast growth factor-23 (FGF-23), a bone derived hormone that acts in the kidney to inhibit phosphate reabsorption and vitamin D biosynthesis. Klotho promotes FGF-23 signaling through binding to FGFRI (IIIc) which converts this canonical FGF receptor into a specific receptor for FGF-23. In the absence of Klotho the function of FGF-23 is literally abolished. Recombinant human Klotho is a 65-70 kDa glycoprotein containing 516 amino acid residues.

Product Categories/Family for KL active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 24497614
• NCBI GeneID: 9365
• NCBI Accession #: NP_004786.2
• NCBI GenBank Nucleotide #: NM_004795.3
• UniProt Accession #: Q9UEF7; Q5VZ95; Q96KV5; Q96KW5; Q9UEI9; Q9Y4F0
• Molecular Weight: 65-70 kDa
• NCBI Official Full Name: klotho
• NCBI Official Synonym Full Names: klotho
• NCBI Official Symbol: KL
• NCBI Protein Information: klotho
• UniProt Protein Name: Klotho
• Protein Family: Klotho
• UniProt Gene Name: KL
• UniProt Entry Name: KLOT_HUMAN

NCBI Description

This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D

By similarity. Essential factor for the specific interaction between FGF23 and FGFR1

By similarity.The Klotho peptide generated by cleavage of the membrane-bound isoform maybe an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling

By similarity.

Catalytic activity: A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

Subunit structure: Homodimer

By similarity. Interacts with FGF23 and FGFR1

By similarity.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note: Isoform 1 shedding leads to a soluble peptide. Ref.4 Ref.7Isoform 2: Secreted Ref.4 Ref.7. Klotho peptide: Secreted Ref.4 Ref.7.

Tissue specificity: Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney. Ref.2 Ref.4 Ref.5 Ref.6 Ref.7

Domain: Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.

Post-translational modification: N-glycosylated

By similarity.

Polymorphism: Homozygosity for KL-VS allele is associated with decreased longevity and increased cardiovascular disease risk.

Involvement in disease: Tumoral calcinosis, hyperphosphatemic, familial (HFTC) [MIM:211900]: A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11

Miscellaneous: Defects in KL may be a cause of chronic renal failure complications.

Sequence similarities: Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.