Rabbit KLOTHO Polyclonal Antibody | anti-KLOTHO antibody

KLOTHO Antibody

Cat. Num. AAA9406108

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: KLOTHO; Polyclonal Antibody; KLOTHO Antibody; KL; anti-KLOTHO antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Three isoforms of KLOTHO are known to exist.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 1012

• Applicable Applications for anti-KLOTHO antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 16 amino acid peptide near the center of human KLOTHO.
• Target Name: KLOTHO
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of KLOTHO in rat heart tissue lysate with KLOTHO antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of KLOTHO in mouse heart tissue with KLOTHO antibody at 2.5 ug/mL.)

Related Product Information for anti-KLOTHO antibody

KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.

Product Categories/Family for anti-KLOTHO antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 24497614
• NCBI GeneID: 9365
• NCBI Accession #: NP_004786
• NCBI GenBank Nucleotide #: NM_004795.3
• Molecular Weight: 62,135 Da
• NCBI Official Full Name: klotho
• NCBI Official Synonym Full Names: klotho
• NCBI Official Symbol: KL
• NCBI Protein Information: klotho
• UniProt Protein Name: Klotho
• Protein Family: Klotho
• UniProt Gene Name: KL
• UniProt Entry Name: KLOT_HUMAN

NCBI Description

This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]

Uniprot Description

Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.31; Membrane protein, integral; Hydrolase

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: extracellular space; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: vitamin D binding; signal transducer activity; fibroblast growth factor binding; beta-glucosidase activity; beta-glucuronidase activity; hormone activity; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; calcium ion homeostasis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; carbohydrate metabolic process; energy reserve metabolic process; insulin receptor signaling pathway; innate immune response; positive regulation of bone mineralization; acute inflammatory response; aging

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial