Testing Data
(Fig-1: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein (MBS669486) and analyzed through In-Cell ELISA using HRP-Streptavidin for detection.)
ACE2 cell line
ACE2/HEK293 Stable Cell Line
Testing Data
(Fig-1: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein (MBS669486) and analyzed through In-Cell ELISA using HRP-Streptavidin for detection.)
Testing Data
(Fig-1: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein (MBS669486) and analyzed through In-Cell ELISA using HRP-Streptavidin for detection.)
Testing Data
(Fig-2: Detection of ACE2 expression in ACE2/HEK293 cells by flow cytometry. ACE2/HEK293 cells were probed with biotinylated SARS-Cov-2 Spike RBD protein (MBS669486) at 1 x 10^5 cells per 1 ug protein on ice for 2 hours. Cells were washed with flow buffer and then incubated with PE-labeled Streptavidin on ice for 20 min. Cell were washed and then analyzed by flow cytometry.)
Testing Data
(Fig-2: Detection of ACE2 expression in ACE2/HEK293 cells by flow cytometry. ACE2/HEK293 cells were probed with biotinylated SARS-Cov-2 Spike RBD protein (MBS669486) at 1 x 10^5 cells per 1 ug protein on ice for 2 hours. Cells were washed with flow buffer and then incubated with PE-labeled Streptavidin on ice for 20 min. Cell were washed and then analyzed by flow cytometry.)
Fig-3: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein (MBS669486) and analyzed by flow cytometry through fluorescent-labeled Streptavidin detection.
Fig-3: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein (MBS669486) and analyzed by flow cytometry through fluorescent-labeled Streptavidin detection.
Testing Data
(Fig-4: Genomic PCR analysis. Genomic DNAs were purified from ACE2/HEK293 stable cells and parental HEK293 cells, and used as the PCR templates. PCR was performed using the target gene construct-specific primer sets, and the plasmid DNA of target gene construct was used as positive control.)
Testing Data
(Fig-4: Genomic PCR analysis. Genomic DNAs were purified from ACE2/HEK293 stable cells and parental HEK293 cells, and used as the PCR templates. PCR was performed using the target gene construct-specific primer sets, and the plasmid DNA of target gene construct was used as positive control.)
Testing Data
(Fig-5: Cell Surface staining of human ACE2 in the ACE2/HEK293 stable cell line. ATTO 488-conjugated anti-hACE2 antibody (clone AC18F; MBS669405) was used at 1ug/ 1x10^6 cells (Red). ATTO 488-conjugated mouse IgG1 was used as isotype control at 1ug/ 1x10^6 cells (Green).)
Testing Data
(Fig-5: Cell Surface staining of human ACE2 in the ACE2/HEK293 stable cell line. ATTO 488-conjugated anti-hACE2 antibody (clone AC18F; MBS669405) was used at 1ug/ 1x10^6 cells (Red). ATTO 488-conjugated mouse IgG1 was used as isotype control at 1ug/ 1x10^6 cells (Green).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
Uniprot Description
ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.17.23; Membrane protein, integral; Protease
Chromosomal Location of Human Ortholog: Xp22
Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; plasma membrane; extracellular region; lipid raft
Molecular Function: peptidyl-dipeptidase activity; viral receptor activity; protein binding; metallopeptidase activity; carboxypeptidase activity; zinc ion binding; peptide hormone binding; endopeptidase activity; glycoprotein binding
Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; proteolysis; angiotensin maturation; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cell proliferation; regulation of cytokine production; cellular protein metabolic process; regulation of vasoconstriction; regulation of inflammatory response; angiotensin catabolic process in blood; receptor biosynthetic process; angiotensin mediated drinking behavior
Research Articles on ACE2
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Product Notes
The ACE2 ace2 (Catalog #AAA669568) is a Cell Line produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ACE2 can be used in a range of immunoassay formats including, but not limited to, Functional Assay (FA). Screen for antibodies of human ACE2 through Flow Cytometry. Protein binding study. . Researchers should empirically determine the suitability of the ACE2 ace2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSSSSWLLLS LVAVTAAQST IEEQAKTFLD KFNHEAEDLF YQSSLASWNY NTNITEENVQ NMNNAGDKWS AFLKEQSTLA QMYPLQEIQN LTVKLQLQAL QQNGSSVLSE DKSKRLNTIL NTMSTIYSTG KVCNPDNPQE CLLLEPGLNE IMANSLDYNE RLWAWESWRS EVGKQLRPLY EEYVVLKNEM ARANHYEDYG DYWRGDYEVN GVDGYDYSRG QLIEDVEHTF EEIKPLYEHL HAYVRAKLMN AYPSYISPIG CLPAHLLGDM WGRFWTNLYS LTVPFGQKPN IDVTDAMVDQ AWDAQRIFKE AEKFFVSVGL PNMTQGFWEN SMLTDPGNVQ KAVCHPTAWD LGKGDFRILM CTKVTMDDFL TAHHEMGHIQ YDMAYAAQPF LLRNGANEGF HEAVGEIMSL SAATPKHLKS IGLLSPDFQE DNETEINFLL KQALTIVGTL PFTYMLEKWR WMVFKGEIPK DQWMKKWWEM KREIVGVVEP VPHDETYCDP ASLFHVSNDY SFIRYYTRTL YQFQFQEALC QAAKHEGPLH KCDISNSTEA GQKLFNMLRL GKSEPWTLAL ENVVGAKNMN VRPLLNYFEP LFTWLKDQNK NSFVGWSTDW SPYADQSIKV RISLKSALGD KAYEWNDNEM YLFRSSVAYA MRQYFLKVKN QMILFGEEDV RVANLKPRIS FNFFVTAPKN VSDIIPRTEV EKAIRMSRSR INDAFRLNDN SLEFLGIQPT LGPPNQPPVS IWLIVFGVVM GVIVVGIVIL IFTGIRDRKK KNKARSGENP YASIDISKGE NNPGFQNTDD VQTSF. It is sometimes possible for the material contained within the vial of "ACE2, Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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