Testing Data
(Figure-1: Antiproliferative activity of Samceptin (Three different Batches) was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit from Promega in SK-BR-3 cells. Result indicated that Samceptin potency is at par with Herceptin.)
ErbB2/HER2 Recombinant Antibody | ERBB2 recombinant antibody
Recombinant anti- human ErbB2/HER2 Antibody (Trastuzumab)
FACS: 1-2 mug/10^6 cells
ADCC: 20-25 mg/ml
Testing Data
(Figure-1: Antiproliferative activity of Samceptin (Three different Batches) was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit from Promega in SK-BR-3 cells. Result indicated that Samceptin potency is at par with Herceptin.)
Testing Data
(Figure-1: Antiproliferative activity of Samceptin (Three different Batches) was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit from Promega in SK-BR-3 cells. Result indicated that Samceptin potency is at par with Herceptin.)
Testing Data
(Figure-2: Antiproliferative activity of Samceptin (Three different Batches) was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit from Promega in MCF-7 cells. Result indicated that Samceptin potency is at par with Herceptin.)
Testing Data
(Figure-2: Antiproliferative activity of Samceptin (Three different Batches) was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit from Promega in MCF-7 cells. Result indicated that Samceptin potency is at par with Herceptin.)
(Figure-3: SEC-HPLC analysis of Herceptin and three batches of Samceptin. Based on this analysis, Samceptin is highly pure and purity estimated to be >98%)
(Figure-3: SEC-HPLC analysis of Herceptin and three batches of Samceptin. Based on this analysis, Samceptin is highly pure and purity estimated to be >98%)
SDS-Page
(Figure-4: Reducing SDS-PGE of three batches of Samceptin in comparison with Herceptin. Lanes were overloaded to show the presence of any other protein bands than Samceptin. Both heavy and light chains are well separated. (Lane-1: Marker, Lane-2: BR-13 Bulk-1 ul, Lane-3: BR-15 Bulk-1 ul, Lane-4: BR-16 Bulk-1 ul, Lane-5: Herceptin-20 ug))
SDS-Page
(Figure-4: Reducing SDS-PGE of three batches of Samceptin in comparison with Herceptin. Lanes were overloaded to show the presence of any other protein bands than Samceptin. Both heavy and light chains are well separated. (Lane-1: Marker, Lane-2: BR-13 Bulk-1 ul, Lane-3: BR-15 Bulk-1 ul, Lane-4: BR-16 Bulk-1 ul, Lane-5: Herceptin-20 ug))
Flow Cytometry (FC/FACS)
(Figure-5: Epitope binding study by flow cytometric analysis. BT-474 cells expressing HER2 antigen were treated with Either Herceptin or Samceptin (1 & 2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
Flow Cytometry (FC/FACS)
(Figure-5: Epitope binding study by flow cytometric analysis. BT-474 cells expressing HER2 antigen were treated with Either Herceptin or Samceptin (1 & 2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
Flow Cytometry (FC/FACS)
(Figure-6: Epitope binding study by flow cytometric analysis. MCF-7 cells expressing HER2 antigen were treated with Either Herceptin or Samceptin (1 & 2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
Flow Cytometry (FC/FACS)
(Figure-6: Epitope binding study by flow cytometric analysis. MCF-7 cells expressing HER2 antigen were treated with Either Herceptin or Samceptin (1 & 2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
Research Articles on ERBB2
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Product Notes
The ERBB2 erbb2 (Catalog #AAA669214) is a Recombinant Antibody produced from CHO and is intended for research purposes only. The product is available for immediate purchase. The Recombinant anti- human ErbB2/HER2 Antibody (Trastuzumab) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ErbB2/HER2 can be used in a range of immunoassay formats including, but not limited to, Functional Assay, Flow Cytometry (FC/FACS). The biological activities of Samceptin was compared with commercially available innovator drug, Herceptin from Roche. FACS: 1-2 mug/10^6 cells ADCC: 20-25 mg/ml. Researchers should empirically determine the suitability of the ERBB2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ErbB2/HER2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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