Test Data
(Immunoprecipitation and Western Blot of Herceptin-2 with (MBS# "Please inquire" and MBS541803. Lane 1,2) IP by MBS541803 and WB with MBS# "Please inquire". Lane 3,4) IP by MBS541803 and WB by MBS541803. Apparent MW is 174-175 kDa. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
Phospho-ErbB 2 Positive Control | ErbB 2 positive control
Phospho-ErbB 2 Positive Control
Test Data
(Immunoprecipitation and Western Blot of Herceptin-2 with (MBS# "Please inquire" and MBS541803. Lane 1,2) IP by MBS541803 and WB with MBS# "Please inquire". Lane 3,4) IP by MBS541803 and WB by MBS541803. Apparent MW is 174-175 kDa. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
Test Data
(Immunoprecipitation and Western Blot of Herceptin-2 with (MBS# "Please inquire" and MBS541803. Lane 1,2) IP by MBS541803 and WB with MBS# "Please inquire". Lane 3,4) IP by MBS541803 and WB by MBS541803. Apparent MW is 174-175 kDa. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
Western Blot Positive Control for Phospho Receptor tyrosine-protein kinase erB-2 (PHER2)
Overview:
The tyrosine kinase receptor erbB2, also known in humans as HER2, is a member of the epidermal growth factor receptor (EGFR or erbB1) family, which also includes erbB3 and erbB4 that are gene products of proto-oncogenes. Protocogenes play an important role in normal proliferation of cells. The interaction of proto-oncogenes with other tumor suppressor proteins (P53, P14ARF etc.) determine the normal or abnormal course of cell cycle and cell division leading to uncontrolled cell proliferation (1). HER2 is found on chromosome 17 and codes for a protein that serves as a cell surface receptor. These HER receptors determine the regulation of cell growth and differentiation in a complex manner. HER family receptors form homo- or hetero- dimers with other compounds. The activation of these homoheterodimmeric complexes by ligand binding leads to activation of intrinsic tyrosine kinase activity that triggers a cascade of signal transduction activity in cell. Some of these signals are transduced across the cell membranes in to cytoplasm and triggers the gene activation. There are at least two humanized recombinant monoclonal antibodies (Trastuzumab and pertuzumab) that target extra cellular regions of the HER2 tyrosine kinase receptor and have been used in human trials for the treatment of breast cancer (2).
Herceptin-2 is a 1342 amino acid protein (approximate MW 170-172 kDa) when analyzed on reduced SOS-PAGE gels. HER2 is over expressed in cells of many cancer types. ErbB receptors bind to a family of growth factors, termed neuregulins/heregulin (NRG/HRG), which comprise NRG-1, -2, -3, and -4 and include multiple variants. HER 2 is a receptor tyrosine kinase with intrinsic tyrosine kinase domain. The Phospho-Her2 antibody (MBS543807) is made against the phospho-Tyrosine containing peptide (aa 1127-1138) from rat HER2 protein.
The PHER2-selective antibodies were generated against unique antigenic sequences form HER2 protein that is not found in any other protein in the gene bank. The Phospho-HER2 antibodies were affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.
Protein type: Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; Oncoprotein; EC 2.7.10.1; TK group; EGFR family
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: apical plasma membrane; basolateral plasma membrane; cytoplasm; cytoplasmic vesicle; endosome membrane; integral to membrane; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; receptor complex
Molecular Function: ATP binding; ErbB-3 class receptor binding; growth factor binding; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein C-terminus binding; protein dimerization activity; protein heterodimerization activity; protein phosphatase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity
Biological Process: cell proliferation; cell surface receptor linked signal transduction; enzyme linked receptor protein signaling pathway; heart development; MAPKKK cascade; motor axon guidance; myelination; negative regulation of immature T cell proliferation in the thymus; neuromuscular junction development; oligodendrocyte differentiation; peptidyl-tyrosine phosphorylation; peripheral nervous system development; phosphoinositide 3-kinase cascade; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of epithelial cell proliferation; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of translation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of angiogenesis; regulation of microtubule-based process; regulation of phosphoinositide 3-kinase cascade; signal transduction; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway; wound healing
Disease: Gastric Cancer; Glioma Susceptibility 1; Lung Cancer
Research Articles on ErbB 2
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Product Notes
The ErbB 2 erbb2 (Catalog #AAA543122) is a Positive Control and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phospho-ErbB 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:500. Researchers should empirically determine the suitability of the ErbB 2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-ErbB 2, Positive Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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