Ribonuclease H Enzyme | Sta7437_3452 enzyme
Ribonuclease H (RNase H)
Purity
Molecular Biology Grade
Synonyms
Ribonuclease H; Ribonuclease H (RNase H); Sta7437_3452 enzyme
Host
E Coli MRE-600 cells
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in 25mM HEPES-KOH, pH 8.0, 50mM KCl, 1mM DTT, 1mM EDTA, 0.1mg/ml BSA, and 50% glycerol.
Application Notes
Suitable for use in Removal of mRNA prior to the synthesis of the 2nd strand of cDNA, Removal of mRNA poly(A) sequences after hybridization with oligo(dT), Site-specific cleavage of RNA and Studies of in vitro polyadenylation rection products.
CAS Number
9050-76-4
Unit Definition
One unit catalyzes the formation of 1nmol of acid soluble products in 20min at 37ºC. Enzyme activity is assayed in the following mixture: 20mM Tris-HCl (H 7.8), 40 mM KCl, 8mM MgCl2, 1mM DTT, 24uM [3H]-POLY(A)-POLY(dT), 0.03mg/ml BSA AND 4% Glycerol.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Sta7437_3452 enzyme
Ribonuclease H specifically degrades only the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded or double-stranded DNA and RNA.
Product Categories/Family for Sta7437_3452 enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
19kD
NCBI Official Full Name
Ribonuclease H
NCBI Official Symbol
Sta7437_3452
NCBI Protein Information
Ribonuclease H
Protein Family
Uniprot Description
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
Similar Products
Product Notes
The Sta7437_3452 (Catalog #AAA653783) is an Enzyme produced from E Coli MRE-600 cells and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in Removal of mRNA prior to the synthesis of the 2nd strand of cDNA, Removal of mRNA poly(A) sequences after hybridization with oligo(dT), Site-specific cleavage of RNA and Studies of in vitro polyadenylation rection products. Researchers should empirically determine the suitability of the Sta7437_3452 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribonuclease H, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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