Testing Data
Thermostable RNase H Enzyme
Thermostable RNase H
Applications
High-stringency hybrid selection and mapping of mRNA structureRemoval of mRNA prior to synthesis of second strand cDNA
Removal of the poly(A) sequences from mRNA in the presence of oligo (dT)
Directed cleavage of RNA
Synonyms
Thermostable RNase H; Thermostable RNase H enzyme
Applicable Applications for Thermostable RNase H enzyme
High-stringency hybrid selection and mapping of mRNA structure
Removal of mRNA prior to synthesis of second strand cDNA
Removal of the poly(A) sequences from mRNA in the presence of oligo (dT)
Directed cleavage of RNA
Removal of mRNA prior to synthesis of second strand cDNA
Removal of the poly(A) sequences from mRNA in the presence of oligo (dT)
Directed cleavage of RNA
Storage Buffer
50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100 and 50% (v/v) Glycerol.
Notes
One unit is defined as the amount of Thermostable RNase H that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA) :poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 ul in 20 minutes at 45 degree C [optimal temperature for poly(rA) :poly(dT) ] in 1X Thermostable RNase H Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 ug poly(dT).
Preparation and Storage
Store all components at -20 degree C.
Testing Data
Testing Data
Related Product Information for Thermostable RNase H enzyme
Thermostable RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. Unlike E Coli RNase H which is inactivated at temperatures above 55 degree C, Thermostable RNase H can withstand much higher temperatures. These higher temperatures allow for higher hybridization stringency for RNA-DNA heteroduplexes resulting in more specific hydrolysis of RNA. Thermostable RNase H has optimal activity above 65 degree C and is active up to 95 degree C making it useful for a broad range of applications.
Similar Products
Product Notes
The Thermostable RNase H (Catalog #AAA4156421) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Thermostable RNase H can be used in a range of immunoassay formats including, but not limited to, High-stringency hybrid selection and mapping of mRNA structure Removal of mRNA prior to synthesis of second strand cDNA Removal of the poly(A) sequences from mRNA in the presence of oligo (dT) Directed cleavage of RNA. Researchers should empirically determine the suitability of the Thermostable RNase H for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thermostable RNase H, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
