Typical Testing Data/Standard Curve (for reference only)
Annexin V Assay Kit | ANXA5 assay kit
Annexin V-EGFP Apoptosis Kit
Fast and convenient
EGFP is a bright and photo-stable green fluorescent protein and the kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: The Annexin V-EGFP Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with an enhanced green fluorescent protein (EGFP) fusion of annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells. Detection can be analyzed by flow cytometry or by fluorescence microscopy with a FITC filter. EGFP is brighter and more photo-stable than other fluorescent reagents. The kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining.
Summary: Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy
Species reactivity- Mammalian
Kit size- 25, 100, 400
Applications-Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]
Uniprot Description
ANXA5: a calcium/phospholipid-binding protein and an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. The binding of labeled ANXA5 to phosphatidylserine is used as a marker of apoptosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
Protein type: Calcium-binding; Inhibitor; Apoptosis; Lipid-binding
Chromosomal Location of Human Ortholog: 4q27
Cellular Component: focal adhesion; membrane; cytoplasm; intracellular; external side of plasma membrane
Molecular Function: phospholipase inhibitor activity; calcium-dependent phospholipid binding; phospholipid binding; calcium ion binding
Biological Process: response to organic substance; negative regulation of catalytic activity; blood coagulation; signal transduction; negative regulation of coagulation; negative regulation of apoptosis
Disease: Pregnancy Loss, Recurrent, Susceptibility To, 3
Research Articles on ANXA5
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Product Notes
The ANXA5 anxa5 (Catalog #AAA841775) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Annexin V, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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