Rabbit anti-Human Natriuretic Peptide Receptor C Polyclonal Antibody | anti-NPRC antibody

Natriuretic Peptide Receptor C, NT (NPRC, NPR-C, Atrial Natriuretic Peptide Receptor Type C, ANP-C, ANPRC, ANPR-C, Atrial Natriuretic Peptide Receptor 3, NPR3, Atrial Natriuretic Peptide Clearance Receptor) (FITC)

Cat. Num. AAA6489409

• Gene Names: NPR3; NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Natriuretic Peptide Receptor C; Polyclonal Antibody; NT (NPRC; NPR-C; Atrial Natriuretic Peptide Receptor Type C; ANP-C; ANPRC; ANPR-C; Atrial Natriuretic Peptide Receptor 3; NPR3; Atrial Natriuretic Peptide Clearance Receptor) (FITC); Natriuretic Peptide Receptor C/Guanylate Cyclase C (Atrionatriuretic Peptide Receptor C); GUCY2B; anti-NPRC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANPC.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NPRC antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ANPC (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8113a Natriuretic Peptide Receptor C (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Natriuretic Peptide Receptor C mainly to the mainly to the nucleus.)

Western Blot (WB)

(Western blot analysis of ANPC-S82 in NCI-H460,HL-60 cell line lysates (35ug/lane). ANPC (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-NPRC antibody

Antibodies; Receptors

References

1. Porter, J.G., et al., Biochem. Biophys. Res. Commun. 171(2):796-803 (1990). 2. Lowe, D.G., et al., Nucleic Acids Res. 18(11), 3412 (1990). 3. Yongjian Liu, et al. J. Nucl. Med., Jan 2010; 51: 85-91. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 324120923
• NCBI GeneID: 4883
• NCBI Accession #: NP_001191305.1
• NCBI GenBank Nucleotide #: NM_001204376.1
• UniProt Accession #: P17342; A2RRD1; B4DT84; E7EPG9
• Molecular Weight: 59,767 Da
• NCBI Official Full Name: atrial natriuretic peptide receptor 3 isoform 3
• NCBI Official Synonym Full Names: natriuretic peptide receptor 3
• NCBI Official Symbol: NPR3
• NCBI Official Synonym Symbols: NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23
• NCBI Protein Information: atrial natriuretic peptide receptor 3
• UniProt Protein Name: Atrial natriuretic peptide receptor 3
• UniProt Gene Name: NPR3
• UniProt Synonym Gene Names: ANPRC; C5orf23; NPRC; ANP-C; ANPR-C; NPR-C

NCBI Description

This gene encodes one of three natriuretic peptide receptors. Natriutetic peptides are small peptides which regulate blood volume and pressure, pulmonary hypertension, and cardiac function as well as some metabolic and growth processes. The product of this gene encodes a natriuretic peptide receptor responsible for clearing circulating and extracellular natriuretic peptides through endocytosis of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.