Western Blot (WB)
(Western Blot: Sample: Recombinant PIK3C2a, Human.)
Rabbit anti-Human Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) Polyclonal Antibody | anti-PIK3C2a antibody
Polyclonal Antibody to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-FNPRMPTFP STEPIYLSLP GQSPYFSYPL TPATPFHPQG SLPIYRPVVS TDMAKLFDKI ASTSEFLKNG KARTDLEITD SKVSNLQVSP KSEDISKFDW LDLDPLSKPK VDNVEVLDHE EEKNVSSLLA KDPWDAVLLE ERSTANCHLE RKVNGKSLSV ATVTRSQSLN IRTTQLAKAQ GHISQKDPNG TSSLPTGSSL
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant PIK3C2a, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant PIK3C2a, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Ovary tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Ovary tissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]
Uniprot Description
PIK3C2A: a protein of the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. Its PI3-kinase is not sensitive to nanomolar levels of the inhibitor wortmannin. Activated by insulin and may be involved in integrin-dependent signaling.
Protein type: Autophagy; Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.154; Kinase, lipid; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: clathrin-coated vesicle; cytoplasm; cytosol; extracellular exosome; Golgi apparatus; membrane; nucleus; phosphoinositide 3-kinase complex; plasma membrane; vesicle
Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; phosphatidylinositol binding; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity
Biological Process: cell migration; clathrin coat assembly; endocytosis; epidermal growth factor receptor signaling pathway; exocytosis; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; phosphatidylinositol phosphorylation; phosphoinositide 3-kinase cascade; platelet-derived growth factor receptor signaling pathway; vascular smooth muscle contraction
Research Articles on PIK3C2a
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Product Notes
The PIK3C2a pik3c2a (Catalog #AAA2016680) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the PIK3C2a pik3c2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-FNP RMPTFP STEPIYLSLP GQSPYFSYPL TPATPFHPQG SLPIYRPVVS TDMAKLFDKI ASTSEFLKNG KARTDLEITD SKVSNLQVSP KSEDISKFDW LDLDPLSKPK VDNVEVLDHE EEKNVSSLLA KDPWDAVLLE ERSTANCHLE RKVNGKSLSV ATVTRSQSLN IRTTQLAKAQ GHISQKDPNG TSSLPTGSSL. It is sometimes possible for the material contained within the vial of "Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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