Rabbit anti-Human, Mouse HUR Polyclonal Antibody | anti-HUR antibody

HUR (ELAVL1, ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-like 1 (Hu Antigen R), ELAV1, ELAV-like Protein 1, Hua, Hu-antigen R, HuR, MelG) (HRP)

Cat. Num. AAA6481265

• Gene Names: ELAVL1; HUR; Hua; MelG; ELAV1
• Reactivity: Human, Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: HUR; Polyclonal Antibody; HUR (ELAVL1; ELAV (Embryonic Lethal; Abnormal Vision; Drosophila)-like 1 (Hu Antigen R); ELAV1; ELAV-like Protein 1; Hua; Hu-antigen R; HuR; MelG) (HRP); anti-HUR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HuR, Mr ~36kD. Species Crossreactivity: mouse
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-HUR antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ICC: 1:500. Detects HuR in HeLa and NIH/3T3 cell lysates.; IHC: Reported to detect HuR in rabbit placental tissue.; IP: 10ug immunoprecipitated HuR in 3T3/A31 RIPA lysates.; Applications are based on unconjugated antibody.; WB: 1ug/ml. Detected HuR in RIPA lysates from 3T3/A31 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with.
• Immunogen: Synthetic peptide corresponding to aa1-13, MSNGYEDHMAEDC, of human HuR, conjugated to KLH (NP_001410). Species sequence homology: monkey (100%)
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Testing Data

(3T3/A31 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with H7953-34. Proteins were visualized using IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates HuR (~36kD).)

Testing Data

(Confocal IF analysis of HeLa, NIH/3T3 using H7953-34 (Red). Actin filaments have been labeled with AlexaFluor 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue).)

Product Categories/Family for anti-HUR antibody

Antibodies; Transcription Factors

References

1. "Localization of the human HuR gene to chromosome 19p13." Hum. Genet. 1997 99(1): 32-33 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 38201714
• NCBI GeneID: 1994
• NCBI Accession #: NP_001410
• NCBI GenBank Nucleotide #: NM_001419
• UniProt Accession #: Q15717; Q53XN6; Q9BTT1
• Molecular Weight: 36,092 Da
• NCBI Official Full Name: ELAV-like protein 1
• NCBI Official Synonym Full Names: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
• NCBI Official Symbol: ELAVL1
• NCBI Official Synonym Symbols: HUR; Hua; MelG; ELAV1
• NCBI Protein Information: ELAV-like protein 1; Hu antigen R; hu-antigen R; embryonic lethal, abnormal vision, drosophila, homolog-like 1
• UniProt Protein Name: ELAV-like protein 1
• Protein Family: Hydroxyurea phosphotransferase
• UniProt Gene Name: ELAVL1
• UniProt Synonym Gene Names: HUR; HuR
• UniProt Entry Name: ELAV1_HUMAN

Uniprot Description

HuR: Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ubiquitous. Belongs to the RRM elav family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus

Molecular Function: mRNA binding; protein binding; mRNA 3'-UTR binding; RNA binding; double-stranded RNA binding; nucleotide binding; AU-rich element binding; protein kinase binding

Biological Process: positive regulation of translation; multicellular organismal development; mRNA stabilization; gene expression