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Western Blot (WB)
(Western Blot; Sample: Recombinant CYP11B1, Rat.)
Rabbit anti-Rat Cytochrome P450 11B1 Polyclonal Antibody | anti-CYP11B1 antibody
Polyclonal Antibody to Cytochrome P450 11B1 (CYP11B1)
Gene Names
Cyp11b1; Cp11ba; RATCP11BA
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Cytochrome P450 11B1; Polyclonal Antibody; Polyclonal Antibody to Cytochrome P450 11B1 (CYP11B1); anti-CYP11B1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CYP11B1 antibody
Westeen Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2071841
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant CYP11B1, Rat.)
Western Blot (WB)
(Western Blot; Sample: Recombinant CYP11B1, Rat.)
Western Blot (WB)
(Western Blot; Sample: Mouse Adrenal gland lysate;Primary Ab: 1ug/ml Rabbit Anti-Rat CYP11B1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Mouse Adrenal gland lysate;Primary Ab: 1ug/ml Rabbit Anti-Rat CYP11B1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Rat Adrenal gland ysate;Primary Ab: 1ug/ml Rabbit Anti-Rat CYP11B1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot; Sample: Rat Adrenal gland ysate;Primary Ab: 1ug/ml Rabbit Anti-Rat CYP11B1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded adrenal tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded adrenal tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Cytochrome P450 11B1, mitochondrial
NCBI Official Synonym Full Names
cytochrome P450, family 11, subfamily b, polypeptide 1
NCBI Official Symbol
Cyp11b1
NCBI Official Synonym Symbols
Cp11ba; RATCP11BA
NCBI Protein Information
cytochrome P450 11B1, mitochondrial
UniProt Protein Name
Cytochrome P450 11B1, mitochondrial
Protein Family
UniProt Gene Name
Cyp11b1
UniProt Synonym Gene Names
Cyp11b-1
NCBI Description
catalyzes the conversion of 11-deoxycorticosterone to corticosterone [RGD, Feb 2006]
Uniprot Description
Forms corticosterone from 11-deoxycorticosterone.
Research Articles on CYP11B1
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Product Notes
The CYP11B1 cyp11b1 (Catalog #AAA2026360) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cytochrome P450 11B1 (CYP11B1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 11B1 can be used in a range of immunoassay formats including, but not limited to, Westeen Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CYP11B1 cyp11b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 11B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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