Rabbit anti-Human DIMT1L Polyclonal Antibody | anti-DIMT1L antibody

DIMT1L, CT (Probable Dimethyladenosine Transferase, DIMT1, HUSSY-05, S-adenosylmethionine-6-N',N'-adenosyl(rRNA) Dimethyltransferase, 18S rRNA Dimethylase, DIM1 Dimethyladenosine Transferase 1-like) (PE)

Cat. Num. AAA6472778

• Gene Names: DIMT1; DIM1; DIMT1L; HUSSY5; HSA9761
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: DIMT1L; Polyclonal Antibody; CT (Probable Dimethyladenosine Transferase; DIMT1; HUSSY-05; S-adenosylmethionine-6-N'; N'-adenosyl(rRNA) Dimethyltransferase; 18S rRNA Dimethylase; DIM1 Dimethyladenosine Transferase 1-like) (PE); anti-DIMT1L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DIMT1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DIMT1L antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human DIMT1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-DIMT1 Pab in T47D cell line lysate (35ug/lane). DIMT1(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-DIMT1L antibody

Antibodies; Enzymes

References

1. Stanchi, F., et al., Yeast 18(1):69-80 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 7657198
• NCBI GeneID: 27292
• NCBI Accession #: NP_055288
• NCBI GenBank Nucleotide #: NM_014473.3
• UniProt Accession #: Q9UNQ2; O76025; Q9BU77; Q9UES1
• Molecular Weight: 37.5kDa (334aa), confirmed by MALDI-TOF
• NCBI Official Full Name: probable dimethyladenosine transferase isoform 1
• NCBI Official Synonym Full Names: DIM1 dimethyladenosine transferase 1 homolog
• NCBI Official Symbol: DIMT1
• NCBI Official Synonym Symbols: DIM1; DIMT1L; HUSSY5; HSA9761
• NCBI Protein Information: probable dimethyladenosine transferase
• UniProt Protein Name: Probable dimethyladenosine transferase
• UniProt Gene Name: DIMT1
• UniProt Synonym Gene Names: DIMT1L
• UniProt Entry Name: DIM1_HUMAN

NCBI Description

The protein encoded by this gene is a methyltransferase that is responsible for dimethylation of adjacent adenosines near the 18S rRNA decoding site. The encoded protein is essential for ribosome biogenesis, although its catalytic activity is not involved in the process. The yeast ortholog of this protein functions in the cytoplasm while this protein functions in the nucleus. [provided by RefSeq, Jan 2017]

Uniprot Description

DIMT1: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.

Protein type: EC 2.1.1.183; Nucleolus; RNA-binding; Methyltransferase

Chromosomal Location of Human Ortholog: 5q12.1

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: rRNA (adenine-N6,N6-)-dimethyltransferase activity

Biological Process: rRNA methylation