Rabbit anti-Human APG7 Polyclonal Antibody | anti-ATG7 antibody

APG7, CT (APG7-Like, APG7L, ATG7 Autophagy-related 7 Homolog (S. cerevisiae), Autophagy Related Protein 7, DKFZp434N0735, GSA7, hAGP7, Ubiquitin Activating Enzyme E1-like Protein)

Cat. Num. AAA643927

• Gene Names: ATG7; APG11; APG7; CVT2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: APG7; Polyclonal Antibody; CT (APG7-Like; APG7L; ATG7 Autophagy-related 7 Homolog (S. cerevisiae); Autophagy Related Protein 7; DKFZp434N0735; GSA7; hAGP7; Ubiquitin Activating Enzyme E1-like Protein); Anti -APG7; anti-ATG7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human ATG7. Species sequence homology: Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-ATG7 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.; Dilution: Western Blot: 0.5-1ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 17aa from near the C-terminal of human APG7 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Liver (formalin-fixed, paraffin-embedded) stained with ATG7 antibody at 5ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-ATG7 antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

Product Categories/Family for anti-ATG7 antibody

Antibodies; Abs to Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 6321965
• NCBI GeneID: 856576
• NCBI Accession #: NP_012041.1
• NCBI GenBank Nucleotide #: NM_001179302.1
• UniProt Accession #: P38862; D3DLC0
• Molecular Weight: 71,428 Da
• NCBI Official Full Name: Atg7p
• NCBI Official Symbol: ATG7
• NCBI Official Synonym Symbols: APG11; APG7; CVT2
• NCBI Protein Information: Atg7p
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: ATG7
• UniProt Synonym Gene Names: APG7; CVT2
• UniProt Entry Name: ATG7_YEAST

Uniprot Description

Function: E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Plays a role in the regulation of filamentous growth and chronological longevity. Ref.1 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.12 Ref.13 Ref.15 Ref.16 Ref.17 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.26 Ref.27 Ref.28 Ref.30

Subunit structure: Homodimer. Interacts with ATG8 through a thioester bond between Cys-507 and the C-terminal 'Gly-116' of ATG8 and with ATG12 through a thioester bond between Cys-507 and the C-terminal 'Gly-186' of ATG12. Interacts also with ATG3. Ref.7 Ref.8 Ref.9 Ref.14 Ref.18 Ref.20 Ref.25 Ref.31

Subcellular location: Cytoplasm. Preautophagosomal structure Ref.6 Ref.7 Ref.19.

Domain: The C-terminal 40 residues are required for homodimerization, as well as the interactions with ATG3, ATG8 and ATG12; and the C-terminal 17 residues are required for the ATG8 lipidation.The GxGxxG motif is important for the function, possibly through binding with ATP.

Sequence similarities: Belongs to the ATG7 family.