Mouse Proteoglycan 4 (PRG4) ELISA Kit | PRG4 elisa kit

Mouse Proteoglycan 4 (PRG4) ELISA Kit

Cat. Num. AAA2706050

• Gene Names: PRG4; MSF; SZP; CACP; HAPO; JCAP
• Reactivity: Mouse
• Synonyms: Proteoglycan 4 (PRG4); Mouse Proteoglycan 4 (PRG4) ELISA Kit; MSF; CACP; HAPO; JCAP; SZP; Megakaryocyte Stimulating factor; Articular Superficial Zone Protein; Camptodactyly; Arthropathy; Coxa Vara; Pericarditis Syndrome; PRG4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Proteoglycan 4 (PRG4).; No significant cross-reactivity or interference between Proteoglycan 4 (PRG4) and analogues was observed.
• Sequence Length: 1,361

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Synovial Fluid and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteoglycan 4 (PRG4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteoglycan 4 (PRG4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRG4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteoglycan 4 (PRG4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteoglycan 4 (PRG4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteoglycan 4 (PRG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteoglycan 4 (PRG4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 735997426
• NCBI GeneID: 10216
• NCBI Accession #: NP_005798.3
• NCBI GenBank Nucleotide #: NM_005807.4
• UniProt Accession #: Q92954; Q6DNC4; Q6DNC5; Q6ZMZ5; Q9BX49
• Molecular Weight: 146,468 Da
• NCBI Official Full Name: proteoglycan 4 isoform A preproprotein
• NCBI Official Synonym Full Names: proteoglycan 4
• NCBI Official Symbol: PRG4
• NCBI Official Synonym Symbols: MSF; SZP; CACP; HAPO; JCAP
• NCBI Protein Information: proteoglycan 4
• UniProt Protein Name: Proteoglycan 4
• Protein Family: Proteoglycan
• UniProt Gene Name: PRG4
• UniProt Synonym Gene Names: MSF; SZP

NCBI Description

The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages.MiscellaneousDifferent forms varying in molecular weight have been observed. Such forms are possibly due to different levels of glycosylation and protein cleavage ().