Goat anti-Mouse Hgfac Polyclonal Antibody | anti-Hgfac antibody

Hgfac (Hepatocyte Growth Factor Activator, HGF Activator, HGFA)

Cat. Num. AAA641899

• Gene Names: Hgfac; HGFA
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Hgfac; Polyclonal Antibody; Hgfac (Hepatocyte Growth Factor Activator; HGF Activator; HGFA); Anti -Hgfac (Hepatocyte Growth Factor Activator; anti-Hgfac antibody

• Host: Goat
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Hgfac.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

• Applicable Applications for anti-Hgfac antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot: 0.1ug/ml; Immunohistochemistry: 5-15ug/ml; Immunoprecipitation: 25ug/ml
• Immunogen: Recombinant corresponding to aa35-653 from mouse Hgfac expressed in NSO cell line.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(HGF Activator in Mouse Intestine. HGF Activator was detected in perfusion fixed frozen sections of mouse intestine using Goat Mouse Pro-HGF Activator Antigen Affinity-purified Polyclonal Antibodyat 15ug/ml overnight at 4°C. Tissue was stained using the Goat HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.)

Related Product Information for anti-Hgfac antibody

Pro-HGF-A is a 96kD member of the PA-FXII-HGFA family of enzymes. It is secreted by hepatocytes and is found in plasma at ~40nM concentration. Other cells selectively reported to express HGF-A include astrocytes, endothelium, fibroblasts and macrophages. Upon activation, HGF-A is known to activate both proHGF and proMSP via proteolytic cleavage. The circulating 96kD form of mouse HGF-A represents an inactive 619aa proform aa35-653. Within this proform exists one FN type II domain aa100-147, an EGF-like domain aa157-195, an FN type III domain aa197-237, a second EGF-like domain aa238-276, a kringle domain aa283-364 and a functional peptidase S1 domain aa406-644. Activation of HGF-A typically occurs with a thrombin-mediated cleavage between aa405-406 and a KLK cleavage between aa369-370. This creates a 34kD heterodimeric active product that contains a 2kD N-terminus aa370-405 disulfide linked to a 32kD C-terminus aa406-653. Once activated, the 34kD heterodimer binds to cell surface HAI-1, rendering it unavailable to circulating HGF. When needed, this HGF-A:HAI-1 complex is released, HGF-A dissociates from HAI-1, and HGF-A is free to activate proHGF. Over aa35-653, mouse HGF-A shares 92% and 82% aa identity with rat and human HGF-A.

Product Categories/Family for anti-Hgfac antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 188219651
• NCBI GeneID: 54426
• NCBI Accession #: NP_062320.2
• NCBI GenBank Nucleotide #: NM_019447.2
• UniProt Accession #: Q9R098; Q9JKV4
• Molecular Weight: 70,568 Da
• NCBI Official Full Name: hepatocyte growth factor activator preproprotein
• NCBI Official Synonym Full Names: hepatocyte growth factor activator
• NCBI Official Symbol: Hgfac
• NCBI Official Synonym Symbols: HGFA
• NCBI Protein Information: hepatocyte growth factor activator; HGF activator
• UniProt Protein Name: Hepatocyte growth factor activator
• Protein Family: Hepatocyte growth factor activator
• UniProt Gene Name: Hgfac
• UniProt Synonym Gene Names: HGF activator; HGFA
• UniProt Entry Name: HGFA_MOUSE

NCBI Description

This gene encodes a serine protease enzyme that proteolytically activates hepatocyte growth factor (HGF) and plays a vital role in the regulation of HGF activity in the regeneration and repair of various tissues. The encoded protein is an inactive zymogen that is proteolytically activated to generate a heterodimeric enzyme consisting of a short chain and a long chain linked by a disulfide bridge. Mice lacking the encoded protein display an impairment in mucosal regeneration after injury. [provided by RefSeq, Jul 2015]

Uniprot Description

HGFAC: Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; EC 3.4.21.-; Protease; Secreted

Cellular Component: extracellular space; rough endoplasmic reticulum; cytoplasm; extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: proteolysis