Rabbit anti-Human EIF4E2 Polyclonal Antibody | anti-EIF4E2 antibody

EIF4E2, NT (Eukaryotic Translation Initiation Factor 4E Type 2, eIF4E Type 2, eIF-4E Type 2, mRNA Cap-binding Protein Type 3, Eukaryotic Translation Initiation Factor 4E-like 3, Eukaryotic Translation Initiation Factor 4E Homologous Protein, mRNA Cap-bind

Cat. Num. AAA6475323

• Gene Names: EIF4E2; 4EHP; IF4e; 4E-LP; EIF4EL3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF4E2; Polyclonal Antibody; NT (Eukaryotic Translation Initiation Factor 4E Type 2; eIF4E Type 2; eIF-4E Type 2; mRNA Cap-binding Protein Type 3; Eukaryotic Translation Initiation Factor 4E-like 3; Eukaryotic Translation Initiation Factor 4E Homologous Protein; mRNA Cap-bind; anti-EIF4E2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EIF4E2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EIF4E2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human EIF4E2 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EIF4E2 Antibody (N-term) polyclonal antibody (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4E2 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-EIF4E2 antibody

Antibodies; Translation Initiation Factors

References

1. Rom, E., et al., J. Biol. Chem. 273(21):13104-13109 (1998). 2. Mao, M., et al., Proc. Natl. Acad. Sci. USA 95(14):8175-8180 (1998). 3. Tee, A.R., et al., FEBS Lett. 564(1-2), 58-62 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4757702
• NCBI GeneID: 9470
• NCBI Accession #: NP_004837
• NCBI GenBank Nucleotide #: NM_004846.2
• UniProt Accession #: O60573; O75349; B8ZZJ9
• Molecular Weight: 27,026 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 4E type 2 isoform A
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E family member 2
• NCBI Official Symbol: EIF4E2
• NCBI Official Synonym Symbols: 4EHP; IF4e; 4E-LP; EIF4EL3
• NCBI Protein Information: eukaryotic translation initiation factor 4E type 2
• UniProt Protein Name: Eukaryotic translation initiation factor 4E type 2
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF4E2
• UniProt Synonym Gene Names: EIF4EL3; eIF-4E type 2; eIF4E type 2
• UniProt Entry Name: IF4E2_HUMAN

Uniprot Description

EIF4E2: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to interact with other partners. EIF4E2 interacts with EIF4EBP1, EIF4EBP2 and EIF4EBP3 but not with eIF4G. Belongs to the eukaryotic initiation factor 4E family.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: mRNA cap complex; cytosol

Molecular Function: protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity; ubiquitin protein ligase binding; RNA cap binding

Biological Process: in utero embryonic development; cytokine and chemokine mediated signaling pathway; negative regulation of translation; translational initiation