Dectin-1 Active Protein | Clec7a active protein

Dectin-1, Recombinant, Human (C-type lectin domain family 7 member A, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Beta-glucan receptor, C-type lectin superfamily member 12, CLEC7A, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082)

Cat. Num. AAA637803

• Gene Names: Clec7a; BGR; beta-GR; Clecsf12
• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Dectin-1; Recombinant; Human (C-type lectin domain family 7 member A; Dendritic cell-associated C-type lectin 1; DC-associated C-type lectin 1; Beta-glucan receptor; C-type lectin superfamily member 12; CLEC7A; BGR; CLECSF12; DECTIN1; UNQ539/PRO1082); Clec7a active protein

• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS.

• Activity: Measured by its ability to bind biotinylated laminarin (1, 3- -glucan) with an estimated KD < 5nM.
• Endotoxin Level: < 0.1 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile phosphate-buffered saline be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for Clec7a active protein

Dectin-1, also known as CLEC7A and the -glucan receptor, is a 33kD type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3-5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8-10). In the membrane, Dectin-1 co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9,11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15-16). The CRD of human Dectin-1 shares 77%, 60%, and 60% aa sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%-39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

Product Categories/Family for Clec7a active protein

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 8102031
• NCBI GeneID: 56644
• UniProt Accession #: Q6QLQ4; Q8K1L4; Q9JI50; E9QPW2
• Molecular Weight: The recombinant human Dectin-1, generated by the removal of the signal peptide region, is a monomeric protein. Based on N-terminal sequencing, the protein begins with the 10X histidine tag and has a calculated molecular mass of approximately 16.8 kD.
• NCBI Official Full Name: dendritic cell-associated C-type lectin-1
• NCBI Official Synonym Full Names: C-type lectin domain family 7, member a
• NCBI Official Symbol: Clec7a
• NCBI Official Synonym Symbols: BGR; beta-GR; Clecsf12
• NCBI Protein Information: C-type lectin domain family 7 member A; dectin-1; beta-glucan receptor; DC-associated C-type lectin 1; C-type lectin superfamily member 12; dendritic cell-associated C-type lectin 1; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12
• UniProt Protein Name: C-type lectin domain family 7 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: Clec7a
• UniProt Synonym Gene Names: Bgr; Clecsf12; Dectin1; DC-associated C-type lectin 1; Dectin-1
• UniProt Entry Name: CLC7A_MOUSE

Uniprot Description

dectin-1: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Up-regulated during differentiation from monocytes into dendritic cells. Isoform 5 interacts with RANBP9. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: zymosan receptor activity; protein binding; opsonin binding; zymosan binding; metal ion binding; carbohydrate binding; pattern recognition receptor activity; polysaccharide binding

Biological Process: immune system process; cell surface pattern recognition receptor signaling pathway; phagocytosis, recognition; cell recognition; response to molecule of fungal origin; pattern recognition receptor signaling pathway; positive regulation of tumor necrosis factor production; cell-cell adhesion; leukocyte activation during immune response; positive regulation of phagocytosis; innate immune response; response to yeast; inflammatory response; phagocytosis, engulfment; detection of yeast